May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Developmental and Tissue Expression Patterns of Mouse Mpp4 Gene
Author Affiliations & Notes
  • M. Li
    Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, CT, United States
  • S.S. Zhang
    Pathology, Yale University School of Medicine, New Haven, CT, United States
  • C.J. Barnstable
    Pathology, Yale University School of Medicine, New Haven, CT, United States
  • Footnotes
    Commercial Relationships  M. Li, None; S.S. Zhang, None; C.J. Barnstable, None.
  • Footnotes
    Support  Grants from the NIH, the Allene Reuss Memorial Trust and the David Woods Kemper Memorial Foundation
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2815. doi:
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      M. Li, S.S. Zhang, C.J. Barnstable; Developmental and Tissue Expression Patterns of Mouse Mpp4 Gene . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2815.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigate the gene expression and regulation mechanisms of mouse retinal development. Methods: Retinal tissue microarray analysis, in situ hybridization on tissue sections, Northern hybridization, RT-PCR, quantitative RT-PCR and Rapid Amplification of cDNA Ends (RACE) were used to identify temporal and spatial gene expression patterns of Mpp4 as well as gene structure in mouse retina and other tissues. Results: Microarray analysis and quantitative RT-PCR showed that mouse Membrane Protein, Palmitoylated 4 (Mpp4), is expressed in the postnatal retina but not in the embryonic retina, with a 50-fold increase in mRNA level in P21 retina vs. P1 retina. In situ hybridization with Mpp4 riboprobes revealed that it is localized exclusively in the photoreceptor cells in P7, P14 and adult retina but is not detectable in P1 retina. It is also localized in pineal gland but not in other regions of the brain by in situ hybridization. Northern hybridization to total RNA with a cDNA probe corresponding to the full-length coding region identified a 2.2kb transcript in adult retina but not in brain, heart, liver or spleen tissues. cDNA cloning and sequencing determined that this 2.2kb transcript, with its sequence identical to the GenBank Mpp4 gene sequence, encodes an N-terminal PDZ domain, a central SH3 domain and a C-terminal GUK domain. Its expression was also detected by RT-PCR at a much lower level in cerebellum, cortex, hippocampus, olfactory bulb and liver tissues. Further RT-PCR analysis with various primers corresponding to different regions of the gene detected that the PDZ and SH3 domains, but not the GUK domain, are expressed at low level in heart and spleen tissues. Conclusions: Mouse Mpp4 gene has been found expressed in postnatal retina, pineal gland and other tissues. Its high level and cell-type specific expression in postnatal retina suggests its important but yet unknown function in photoreceptor development.

Keywords: retinal development • gene microarray • in situ hybridization 
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