May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Cytokine Influence on the mRNA of Cell Cycle and ECM Proteins by hRPE Cells In vitro
Author Affiliations & Notes
  • M. Hollborn
    Dept. of Ophthalmology, University of Leipzig, Leipzig, Germany
  • P. Wiedemann
    Dept. of Ophthalmology, University of Leipzig, Leipzig, Germany
  • L. Kohen
    Dept. of Ophthalmology, University of Leipzig, Leipzig, Germany
  • Footnotes
    Commercial Relationships  M. Hollborn, None; P. Wiedemann, None; L. Kohen, None.
  • Footnotes
    Support  DFG Grant Ko 1547/4-1
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 2948. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M. Hollborn, P. Wiedemann, L. Kohen; Cytokine Influence on the mRNA of Cell Cycle and ECM Proteins by hRPE Cells In vitro . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2948.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: The retinal pigment epithelial cells (RPE) play a pivotal role in the pathogenesis of the proliferative vitreoretinopathy (PVR). Changes of the concentrations of growth factors involved in the pathogenesis of PVR seem to induce the re-entering of different retinal cell types into the cell cycle. The aim of these investigations was to analyse the effects of growth factors on the proliferation of hRPE cells and on the mRNA expression of transcription factors, cell cycle proteins and extracellular matrix (ECM) proteins. Methods: Human RPE cells were incubated in the presence of TGFß1, TGFß2, PDGF, VEGF or bFGF and cell proliferation was determined by the BrdU incorporation after 24 - 72h. The changes in mRNA expression of c-fos, c-myc, PCNA, FEN1, Ki67, collagen III and collagen IV were investigated using the ribonuclease protection assay (RPA). Results: RPE cell proliferation was significant increased by PDGF and bFGF after 48h and decreased by TGFß1 and TGFß2 after 48 - 72h. All growth factors elevated significantly c-fos mRNA amount after 1h as c-myc mRNA after 24h. Effects on the mRNA expression of cell cycle proteins were observed significantly by bFGF after 24h stimulation. PDGF and bFGF upregulated the Ki67 mRNA expression and downregulated the mRNA of coll III and coll IV. TGFß1 and TGFß2 decreased the Ki67 mRNA expression and increased the coll III and coll IV mRNA amount. Conclusions: These results show that some of the examined cytokines induce contrary effects in respect of proliferation and extracellular matrix formation by hRPE cells in vitro. This knowledge may be used for therapeutical approaches.

Keywords: retinal pigment epithelium • proliferation • gene/expression 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×