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K.H. Eibl, C.L. Schoenfeld, C.A. May, A.S. Neubauer, S. Priglinger, A. Kampik, U. Welge-Lussen; Oleyl-Phosphocholine Inhibits Proliferation of Human RPE Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):2957.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To determine the effect of Oleyl-Phosphocholine (C18:1-PC) on human retinal pigment epithelium (RPE) proliferation and RPE mediated collagen matrix contraction in vitro. Methods: Cultured RPE cells of five human donors were treated with C18:1-PC in the presence of fetal calf serum. Proliferation was assessed by the tetrazolium dye-reduction assay (MTT). The effect of C18:1-PC on RPE mediated three-dimensional collagen gel contraction was determined. Cell viability was tested by the trypan blue exclusion assay. Results: C18:1-PC inhibited RPE proliferation and RPE mediated collagen matrix contraction in vitro. The antiproliferative and anticontractile effect of C18:1-PC increased in a dose-dependent manner. The IC50 concentrations of C18:1-PC was 26.5 µM ±1.3. Trypan blue staining revealed a toxicity below 5 % within the concentration interval tested. Conclusions: C18:1-PC inhibits proliferation of RPE cells and RPE-mediated matrix contraction in vitro at non-toxic concentrations. This could be a new strategy for prevention of RPE-mediated proliferative processes such as PVR.
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