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A. Maia, C. Muccioli, A.F. Teixeira; Retinal Vascular Cannulation Pig Cadaver Eye Model . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3020.
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Purpose: Develop new surgical technique that might be effective in restoring blood flow in retinal vascular occlusions. We investigate the surgical technique to (1) gain access into the retinal vasculature (2) cannulate retinal blood vessels with a rigid microcannula (3) inject liquid into retinal vein. Methods: Pig cadaveric eyes were submitted to pars plana lensectomy and to pars plana vitrectomy. Trocar system plastic made was placed in the sclerotomy to avoid capillary break. Glass made microcapillary used for transferring embryonic stem (ES) cells – Eppendorf – Germany, attached to Accurus (VFC), were used to inject fluorescein into central retinal vein. Capillaries had 15 micra (inner diameter) and a 20o angle. Results: The tecnique described was able to inject fluorescein into central vein of cadaveric pig eyes, for a short period of time. Reflux towards periphery was observed due to abscence of normal blood flow. Capilaries are resistent to manipulation. For longer injections, stabilization system is required. Conclusions: Retinal vein canalization is a feaseble procedure. Whether this technique will prove beneficial and safe in the treatment of central retinal vein occlusion remains to be determined by animal and clinical studies. View OriginalDownload SlideView OriginalDownload Slide
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