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C. Valmaggia, B. Willekens, M. de Smet; Microplasmin Induced Vitreolysis in Porcine Eyes . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3050.
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Purpose: Microplasmin is a low molecular weight recombinant human protein comprising the catalytic domain of plasmin. Its catalytic activity is similar to that of full length plasmin. Plasmin has been previously shown to be of benefit in inducing a pharmacological vitreous separation. The current experiments were aimed at demonstrating the effect of microplasmin on the vitreous structure and interface in a porcine eye model. Methods: Microplasmine at different concentrations of 0.0625 mg to 0.39 mg in 0.1 ml was injected via the pars plana in pigs eyes immediately after death. Bovine plasmin with concentration of 2 U in 0.1 ml was used in a control group. At time 0, 15 ,30 , 60 and 120 min after injection, the eyes were fixed in Peter's solution and placed in ice to stop further enzymatic activity. The eyes were carefully dehydrated, prepared and examined with conventional and electronic microscopy at magnifications up to 12000x. Results: Vitreo-retinal adhesions were markedly diminished out to the ora when incubation was carried out with Microplasmine concentrations equal or above 0.125 mg. There were also significant changes to the fibrillar structure of the vitreous body. Conclusions: Microplasmine weakens the vitreous adhesion in porcine eyes. Further studies regarding the safety of Microsplamine are needed before studies in humans may be considered.
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