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C.Y. Chan, C.H. To, A. Zamudio, O.A. Candia; Measurement of Aqueous Humor Formation Rate Using a Modified Ussing-Type Chamber by Capillary Method . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3430.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose. To measure the aqueous humor formation (AHF) rate in bovine ciliary body epithelium (CBE) using a modified Ussing-type chamber. Methods. Whole bovine CBE was isolated and sandwiched between a modified Ussing-type chamber. These chambers were open re-circulating type chambers with Ringer reservoir on one side and capillary tube on the other. Changes of capillary level with time allowed the calculation of AHF rate. The chamber was kept at 33-36°C by heating coils. Effects of chloride substitution and ouabain were examined. Results. The rate of in vitro AHF was 1.12 ± 0.13 µl/hr/cm2. Addition of ouabain (1mM) significantly reduced the formation rate by 55%. Chloride-free bathing solution at stromal side also significantly reduced the AHF rate. Conclusion. We have measured the in vitro AHF rate in bovine CBE using a modified Ussing-type chamber. The bovine in vitro AHF was shown to be largely active and depended on chloride ion concentration.
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