May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Measurement of Aqueous Humor Formation Rate Using a Modified Ussing-Type Chamber by Capillary Method
Author Affiliations & Notes
  • C.Y. Chan
    Department of Optometry & Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • C.H. To
    Department of Optometry & Radiography, The Hong Kong Polytechnic University, Hong Kong, Hong Kong Special Administrative Region of China
  • A. Zamudio
    Department of Ophthalmology, Mount Sinai School of Medicine, New York, NY, United States
  • O.A. Candia
    Department of Ophthalmology, Mount Sinai School of Medicine, New York, NY, United States
  • Footnotes
    Commercial Relationships  C.Y. Chan, None; C.H. To, None; A. Zamudio, None; O.A. Candia, None.
  • Footnotes
    Support  The Hong Kong Polytechnic University Research Grant GT251.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3430. doi:
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      C.Y. Chan, C.H. To, A. Zamudio, O.A. Candia; Measurement of Aqueous Humor Formation Rate Using a Modified Ussing-Type Chamber by Capillary Method . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3430.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose. To measure the aqueous humor formation (AHF) rate in bovine ciliary body epithelium (CBE) using a modified Ussing-type chamber. Methods. Whole bovine CBE was isolated and sandwiched between a modified Ussing-type chamber. These chambers were open re-circulating type chambers with Ringer reservoir on one side and capillary tube on the other. Changes of capillary level with time allowed the calculation of AHF rate. The chamber was kept at 33-36°C by heating coils. Effects of chloride substitution and ouabain were examined. Results. The rate of in vitro AHF was 1.12 ± 0.13 µl/hr/cm2. Addition of ouabain (1mM) significantly reduced the formation rate by 55%. Chloride-free bathing solution at stromal side also significantly reduced the AHF rate. Conclusion. We have measured the in vitro AHF rate in bovine CBE using a modified Ussing-type chamber. The bovine in vitro AHF was shown to be largely active and depended on chloride ion concentration.

Keywords: aqueous • ciliary body 
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