May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Raman Probes for Non-Invasive Analysis of the Anterior Chamber in the Eye
Author Affiliations & Notes
  • R.J. Erckens
    Ophthalmology, University Hospital Maastricht, Maastricht, Netherlands
  • F.H. Jongsma
    Ophthalmology, University Hospital Maastricht, Maastricht, Netherlands
  • J. de Brabander
    Ophthalmology, University Hospital Maastricht, Maastricht, Netherlands
  • G.J. Puppels
    Surgery, Erasmus University Rotterdam, Rotterdam, Netherlands
  • F. Hendrikse
    Surgery, Erasmus University Rotterdam, Rotterdam, Netherlands
  • Footnotes
    Commercial Relationships  R.J. Erckens, None; F.H.M. Jongsma, None; J. de Brabander, None; G.J. Puppels, None; F. Hendrikse, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3438. doi:
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      R.J. Erckens, F.H. Jongsma, J. de Brabander, G.J. Puppels, F. Hendrikse; Raman Probes for Non-Invasive Analysis of the Anterior Chamber in the Eye . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3438.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To develop probes for non-invasive Raman spectroscopic analysis of aqueous humor with no danger to the retinal tissue of the probing beam. Methods: Obtaining Raman spectra with a safe light dose require that the excitation laser light is below the retinal light exposure damage threshold. This can be achieved by exciting at an angle through the corneal tissue. However this results in optical aberrations, especially astigmatism, that reduce the sensitivity of the technique and the resolution of the spectra. Two probes were developed, a confocal probe and an optical dissection probe. The latter may also be used to perform infrared absorption spectroscopy. Results: With the special designed probes the optical aberrations are prevented. Conclusions: These probes utilized in a Raman spectroscopic system provide spectra with a resolution and sensitivity comparable with in vitro obtained spectra.

Keywords: aqueous • anterior chamber • optical properties 
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