Purchase this article with an account.
F. Hoffmann, E. Zhang, A. Mueller, R. Ignatius; Significant Prolongation of Orthotopic Corneal Graft Survival in Fty720-Treated Mice . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3450.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose: The novel immunomodulator, FTY720, mainly acts through sequestering of lymphocytes to secondary lymphatic tissue, thereby suppressing their infiltration into grafted organs. This study aimed to investigate its influence on corneal graft survival. Methods: Sixteen BALB/c mice (H-2d) received corneal transplants from C3H (H-2k) mice. Eight mice were treated with FTY720 (10 mg/kg/d) orally from day –1 to day 11, all animals received 0.1% dexamethasone eye drops for the same time. Additionally, eyes and regional lymph nodes from similarly treated animals were subjected to immunohistochemistry and proliferation assays 29-31 days after transplantation. Results: FTY720 significantly prolonged graft survival from 28±8.1 to 36.5±7.1 days (p=0.021). In treated animals corneal infiltration by CD4+ and F4/80+ cells was reduced from 70.8±60.3 to 7.0±9.0 (p=0.004) and from 97.5±30.7 to 44.8±24.9 (p=0.01) cells, respectively, and allogeneic T cell proliferation of regional lymph node cells towards gamma irradiated donor spleen cells was decreased to some 43%. Conclusions: FTY720 treatment substantially protects corneal allografts and may provide an immunomodulatory strategy in clinical corneal transplantation.
This PDF is available to Subscribers Only