May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
DNA Microarray Analysis of Lens Epithelial Cells in Selenite-injected Rats
Author Affiliations & Notes
  • T. Nakajima
    Senju Laboratory of Ocular Sciences, Senju Pharmaceutical Co., Ltd., Beaverton, OR, United States
  • P.B. Belusko
    Senju Laboratory of Ocular Sciences, Senju Pharmaceutical Co., Ltd., Beaverton, OR, United States
  • M. Azuma
    Senju Laboratory of Ocular Sciences, Senju Pharmaceutical Co., Ltd., Beaverton, OR, United States
  • T.R. Shearer
    Department of Oral Molecular Biology, Oregon Health & Science University, Portland, OR, United States
  • Footnotes
    Commercial Relationships  T. Nakajima, None; P.B. Belusko, None; M. Azuma, None; T.R. Shearer, Senju Pharmaceutical Co., Ltd. C.
  • Footnotes
    Support  NIH grant EY03600
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3504. doi:
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      T. Nakajima, P.B. Belusko, M. Azuma, T.R. Shearer; DNA Microarray Analysis of Lens Epithelial Cells in Selenite-injected Rats . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3504.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Apoptosis in the lens epithelial cells was previously shown to be a contributing factor in the selenite-induced cataract. Further, microarray analysis indicated that expression of mRNAs for cytochrome c oxidase (COX-I) and early growth response protein-1 (EGR-1) involved in apoptosis were significantly changed. Thus, the purposes of the present study were: 1) to reconfirm changes in the gene expression, and 2) to broaden the search for other genes altered in lens epithelial cells from selenite-injected rats. Methods: Lens epithelial cells were harvested at 1 and 2 days after a single subcutaneous injection of sodium selenite (30 µmol/kg body weight) into 12-day-old-rats. Gene expression was analyzed using a commercial DNA array (Rat Genome U34A GeneChip array, Affymetrix). Changes in the expression patterns of mRNAs were confirmed by RT-PCR. Results: Of approximately 8000 genes assayed by hybridization, 14 genes were decreased and 28 genes were increased in the rat lens epithelial cells after injection of selenite. Some of the up-regulated genes included apoptosis-related genes, and a majority of the down-regulated genes were mitochondrial genes. Previously observed changes in expression of mRNAs such as EGR-1 were also confirmed. Conclusions: Our data identified several novel genes in addition to obvious genes that may be important for alteration in metabolism and the induction of apoptosis in the lens epithelial cells from selenite-injected rats. Such changes may lead to formation of selenite cataract.

Keywords: cataract • molecular biology • cell death/apoptosis 
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