May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Eotaxin-1 Drives Terminal Differentiation of Conjunctival Mast Cells
Author Affiliations & Notes
  • T. Nakamura
    Ocular Immunology, University College London, London, United Kingdom
  • M. Toda
    Ocular Immunology, University College London, London, United Kingdom
  • D. Miyazaki
    Ocular Immunology, University College London, London, United Kingdom
  • M.E. Rothenberg
    Allergy/Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States
  • S.J. Ono
    Allergy/Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States
  • Footnotes
    Commercial Relationships  T. Nakamura, Santen F; M. Toda, None; D. Miyazaki, None; M.E. Rothenberg, None; S.J. Ono, Santen C, R.
  • Footnotes
    Support  NIH EY1901, Fight for Sight, Santen Inc. and JSPS
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3753. doi:
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      T. Nakamura, M. Toda, D. Miyazaki, M.E. Rothenberg, S.J. Ono; Eotaxin-1 Drives Terminal Differentiation of Conjunctival Mast Cells . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3753.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Eotaxin-1 is a member of the C-C chemokine subfamily that is expressed in a wide variety of mesenchymal cells and plays an important role in the late phase reaction as an eosinophil chemoattractant. We have previously reported that eotaxin-1 is also essential for the acute phase reaction in the conjunctiva, based upon analyses of eotaxin-1-deficient mice. We report here that eotaxin-1 serves to prime and drive terminal differentiation of mast cells in the conjunctiva. Methods and Results: Passive IgE-sensitization experiments using conjunctival mast cells from eotaxin-1-deficient mice has revealed that addition of recombinant eotaxin-1 rescues the impaired mast cell degranulation observed in these mice, while having no effect on conjunctival mast cells from wild-type mice. These data demonstrate that eotaxin-1 can prime conjunctival mast cells to respond to FceRI cross-linking in a remarkably rapid fashion. The evidence that this priming involves terminal differentiation of the mast cells comes from our finding that eotaxin-1-deficient mice exhibited decreased mRNA expression of mouse mast cell proteases -5 and -6 in the conjunctiva compared with those from wild-type mice. Conclusions: Our current work aims to decipher CCR3-mdeiated signal transduction pathways which modulate expression of mast cell proteases during terminal differentiation. We hope that these experiments will provide a molecular basis for the role eotaxin-1 plays in mast cell development and function.

Keywords: cytokines/chemokines • conjunctiva • conjunctivitis 
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