May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Prospective Variation in the Expression of the Conjunctival Mucin Genes Muc5ac, Muc7, Muc16, and Muc17 Before, During and After Contact Lens Wear
Author Affiliations & Notes
  • R.M. Corrales
    Ioba, University of Valladolid, Valladolid, Spain
  • D.J. Galarreta
    Ioba, University of Valladolid, Valladolid, Spain
  • J.M. Herreras
    Ioba, University of Valladolid, Valladolid, Spain
  • M.J. González
    Ioba, University of Valladolid, Valladolid, Spain
  • I. Arranz
    Ioba, University of Valladolid, Valladolid, Spain
  • V. Saez
    Ioba, University of Valladolid, Valladolid, Spain
  • A. Mayo
    Ioba, University of Valladolid, Valladolid, Spain
  • C. García-Vázquez
    Ioba, University of Valladolid, Valladolid, Spain
  • M. Calonge
    Ioba, University of Valladolid, Valladolid, Spain
  • F.J. Chaves
    Endocrinology, Hospital Clínico Universitario, Valencia, Spain
  • Footnotes
    Commercial Relationships  R.M. Corrales, None; D.J. Galarreta, None; J.M. Herreras, None; M.J. González, None; I. Arranz, None; V. Saez, None; A. Mayo, None; C. García-Vázquez, None; M. Calonge, None; F.J. Chaves, None.
  • Footnotes
    Support  FIS-01/0185
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3777. doi:
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      R.M. Corrales, D.J. Galarreta, J.M. Herreras, M.J. González, I. Arranz, V. Saez, A. Mayo, C. García-Vázquez, M. Calonge, F.J. Chaves; Prospective Variation in the Expression of the Conjunctival Mucin Genes Muc5ac, Muc7, Muc16, and Muc17 Before, During and After Contact Lens Wear . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3777.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:Little is known about the potential of contact lens wear to induce a dry eye syndrome by altering the conjunctival mucins. This study analyze the variation of the conjunctival mucin genes expression published (MUC1, MUC2, MUC4, MUC5AC) in addition to others not published yet (MUC7, MUC15, MUC16, MUC17) in healthy subjects before, during, and after hydrogel contact lenses (HCL) wear for 1 year. Methods:Two polyethersulfone filters were applied to the superior bulbar conjunctiva of both eyes of 8 healthy donors who never had worn contact lenses before, 6 months, and 1 year after HCL fitting, and 15 days after discontinuation. Real time PCR was performed using total RNA to quantite the expression of ocular mucin genes. Their relative quantity was determined by SYBR® Green I dye detection. The housekeeping gene GAPDH was used to normalize the amount of expression levels. Chronologic variations were analyzed using Student's test and lineal regression. Results: Transcripts of MUC1, MUC2, MUC4, MUC5AC, MUC7, MUC15, MUC16, and MUC17 were detected in all healthy subjects just before HCL fitting. Changes in the expression of MUC1, MUC2, MUC4 and MUC15 were not significant after 6 or 12 months of HCL wearing. However, the number of transcripts for MUC5AC, MUC16, and MUC17 (p < 0.05) significantly decreased, whereas MUC7 significantly increased (p<0.0001) after 12 months of HCL wear. There were not significant changes in the expression of conjunctival mucin genes 15 days after discontinuing HCL wear. Conclusions: This study is the first to report changes induced by HCL wear in a wide range of conjunctival mucin genes, also analyzing mucin genes not previously reported in the human conjunctival epithelium. These findings may offer additional etiopathogenic factors to be considered in the development of contact lens-induced dry eye syndrome.

Keywords: cornea: surface mucins • contact lens • conjunctiva 
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