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P.O. Lafontaine, N. Buron, M. Soulie, E. Solary, A.M. Bron, C. Garcher; ARNm Expression of TFF1 Peptide after Refractive Surgery . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3778.
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Purpose: Trefoil Factors 1 (TFF1) belong to trefoil factor family, typical secretory products of mucous epithelia. TFF are involved in gastric wound healing. They are co-expressed with mucins and act in concert with them. Our aim was to assess the kinetics of TFF1 mRNA expression during the corneal wound healing due to PRK (Photo Refractive Keratectomy). Methods: PRK was performed on 6 patients (Age: 24-38) for myopia (-1.75 to -4.25 D). Impression cytology specimens were collected before surgery and 1 hour, 1, 3, 5 and 7 days following surgery. We assessed the expression of TFF1 mRNA by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). In the same time, repeated photographs measured wound-healing areas. Results: No correlation was found between TFF1 mRNA expression and wound healing kinetic. Conclusions: TFF1 mRNA expression was increased in response to corneal ulceration. An abnormal regulation of TFF secretion could explain the delayed wound healing observed in ocular surface disease occurring after PRK. Mean of TFF1 expression (arbitrary units) View OriginalDownload SlideView OriginalDownload Slide
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