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F.D. Raiskup, E. Binstock, A. Domb, J. Frucht-Pery; Iontophoresis (Mini ION) for Corticosteroid Administration into the Rabbit Cornea . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3846.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate iontophoresis induced penetration of dexamethasone phosphate (DMF) into the rabbit eye. Methods: 40 rabbits underwent general anesthesia (inj. Ketamine and inj. Xalazine i.m.) and were divided into six groups. Three groups of 8 rabbits each (one eye/rabbit) underwent corneal iontophoresis using Mini ION system with hydrogel disc probe containing DMF. Current of 1 mAmp, applied to center of cornea for 1 min (group 1), 2 min (group 2) and 2x2 min (group 3). 8 rabbits (group 4) received drops of DMF 0.1% at 5 min intervals for 1 hour. Groups 5 (n=4) and 6 (n=4) received iontophoresis (2x2min) transconjunctivally or transclerally correspondingly. Animals were sacrificed within 20 min after the treatment and concentrations of DMF in cornea and aqueous humor (groups 1-4) or vitreous body and retina (groups 5 and 6) were detected by HPLC method. Results: Mann-Whitney test with Bonferroni correction used for statistical analysis. The concentrations of DMF in corneas (µg/gr): group 1 (1363.7± 436.3), group 2 (1187.6±742.0), group 3 (3077.5±1138.9) were higher (p=0.001) than in group 4 (46.9±10.5). The levels of DMF in aqueous humor (µg/ml) in groups 1-3 (19.7±10.0; 21.9±14.1; 19.9±18.4) were not different from group 4(18.2±4.0). In group 5 329.0±103.7 µg/gr and in group 6 341.4±156.7 µg/gr of DMF were found in retina. In vitreous body the levels of DMF were 5.2±2.4 µg/ml in group 5 and 19.2±32.5µg/ml in group 6. Conclusions: Use of iontophoresis can achieve high concentrations of DMF in the rabbit cornea and retina.
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