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J.M. Lopes De Faria, T.C. Correa, A.V. Ferrari, J.B. Lopes de Faria; Genetic Susceptibility to Development of Severe Forms of Diabetic Retinopathy. Possible Mechanisms of Interactions with Hypertension . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3904.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigate the effects of DM on neuro retinal cell replication and on vascular permeability in SRH and their normotensive control WKY rats. Methods: Diabetes was induced by an intravenous injection of streptozotocin in SHR and WKY with 4 and 12 weeks old.After 2 weeks of DM, the animals were sacrificed,the eyes enucleated, fixed in paraformaldehyde and embedded in paraffin. The retinas were used for BrDU immunohystochemical or for protein extraction for p27 Western blot assays.To evaluate retina vascular permeability, a quantitative method using Evans Blue was performed. Results: Neural cell proliferation was lower in diabetic SHR than in control SHR and in diabetic and control WKY rats (0 vs 0.031vs 0.042vs 0.042, respectively, p=0.001) with 4 and 12 weeks of age (0.025vs 1.18vs 0.091vs 0.149, respectively, p=0.001). The p27 retinal proteins levels were higher in SHR after induction of DM. There was a breakdown of retinal permeability in diabetic rats, mainly in SHR. Conclusion: DM inhibites retinal neural cell proliferation, and the hypertension amplifies this effect, possible by increasing the p27 levels in the retina. The vascular permeability may be influenced by the neuro retinal elements.
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