May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Apoptosis in Acute Photodynamic Therapy of Choroidal Neovascularization in the Rat Model
Author Affiliations & Notes
  • T.A. Young
    Retina Service, Vision Science Research Program, University of Toronto, Canada; Angiogenesis Laboratory, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA, United States
  • M.B. Pantcheva
    Ophthalmology, Howe Laboratory, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA, United States
  • E.J. Connolly
    Retina Service, Angiogenesis Laboratory, Mass Eye & Ear Infirmary, Harvard Medical School, Boston, MA, United States
  • M.S. Mandelcorn
    Ophthalmology, University of Toronto, Toronto, ON, Canada
  • C.I. Whiteside
    Medicine, University of Toronto, Toronto, ON, Canada
  • C.L. Grosskreutz
    Medicine, University of Toronto, Toronto, ON, Canada
  • E.S. Gragoudas
    Retina Service, Angiogenesis Laboratory, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA, United States
  • J.W. Miller
    Retina Service, Angiogenesis Laboratory, Massachusetts Eye & Ear Infirmary, Harvard Medical School, Boston, MA, United States
  • Footnotes
    Commercial Relationships  T.A. Young, None; M.B. Pantcheva, None; E.J. Connolly, None; M.S. Mandelcorn, None; C.I. Whiteside, None; C.L. Grosskreutz, None; E.S. Gragoudas, Massachusetts Eye and Ear Infirmary P; J.W. Miller, Massachusetts Eye and Ear Infirmary P.
  • Footnotes
    Support  Vision Science Research Program, Foundation Fighting Blindness
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 3932. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      T.A. Young, M.B. Pantcheva, E.J. Connolly, M.S. Mandelcorn, C.I. Whiteside, C.L. Grosskreutz, E.S. Gragoudas, J.W. Miller; Apoptosis in Acute Photodynamic Therapy of Choroidal Neovascularization in the Rat Model . Invest. Ophthalmol. Vis. Sci. 2003;44(13):3932.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Photodynamic therapy (PDT) using verteporfin is an accepted therapy of choroidal neovascularization (CNV). Apoptosis has been postulated as a mechanism by which PDT is effective in tumors and other disease entities. We have previously shown that modulation of caspase and Bcl-2 family occurs after PDT of microvascular endothelial and retinal pigment epithelial cells in vitro. The purpose of this work was to investigate the role of apoptosis in vivo following verteporfin PDT of choroidal neovascularization in the rat. Methods: Laser burns were applied in a peripapillary fashion in 30 eyes of Brown-Norway rats and CNV was documented by angiographic leakage 3-4 weeks after laser. Verteporfin PDT was applied to leaking CNV using 6 mg/m2 verteporfin followed by laser irradiation using 600 mW/cm2 and 25 J/cm2 at 689 nm 15 minutes after drug injection. Eyes were enucleated at 1 h, 2h, 4 h, and 24 h after treatment. TUNEL staining performed on sections after paraformaldehyde fixation and paraffin embedding was performed to evaluate retinal apoptosis of treated lesions. Results: TUNEL positive cells were identified in PDT treated lesion sites at all time points of specimen sections. Positive stained cells were observed in the outer nuclear layer and endothelial cells comprising the CNV lesion, in only PDT treated eyes. CNV lesions which did not receive PDT did not show TUNEL posivitity. Conclusions: Apoptosis may play an important role in the mechanism of action of PDT treated CNV lesions. Signals for the occurrence of subsequent leaking vessel closure may be mediated by acute apoptotic activity post verteporfin PDT.

Keywords: age-related macular degeneration • photodynamic therapy • apoptosis/cell death 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×