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G.L. Palacio, M. Maia, L. Kellner, E. de Juan, R. Smith, M.E. Farah, E. Margalit, L. Grebe, K.G. Au Eong, M.S. Humayun; Effects of Indocyanine Green Delivery over the Retinal Surface and into the Subretinal Space of Rabbits . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4066.
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Purpose:To evaluate the effects of indocyanine green (ICG) injection on the retinal surface and into the subretinal space of rabbit eyes.Methods:Twenty-two dutch belted rabbits were submitted to a two-port vitrectomy followed by 5mg/ml of ICG delivery over the retinal surface and into the subretinal space. Balanced salt solution (BSS) was also injected subretinally. The retina where the ICG was injected epiretinally and in one site subretinally were exposed to light from an endoilluminator for 7 minutes. The animals were examined at 1, 7 and 14 days after surgery. Eyes were studied by fluorescein angiography, light and electron microscopy. Results:No damage was observed after ICG injection over the retinal surface but the injection of this dye into the subretinal space resulted in damage of the outer nuclear layer (ONL), photoreceptors segments (PS) and retinal pigment epithelium (RPE). This damage was more severe on longer intervals of follow-up. Control experiments in which BSS was injected into the subretinal space or light was delivered over the retina demonstrated only photoreceptors outer segments damage.Conclusions:Subretinal delivery of 5mg/ml of ICG induces RPE, PS and ONL damage; when exposed to light, an additional injurious photodynamic effect may also be present.
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