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M. Maia, E. Margalit, R. Grebe, E. de Juan, K.G. Au Eong, M.E. Farah, M.O. Tso, J. Weiland, G. Fujii, M.S. Humayun; Effects of Intravitreal Indocyanine Green Injection in Rabbits . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4445.
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Purpose:To report clinical, electrophysiological and histological findings of different concentrations of indocyanine green (ICG) injected into the vitreous cavity of rabbit eyes.Methods:Forty-two rabbits had intravitreal injection of 0.1ml of ICG in three different concentrations: 0.5mg/ml (250mOsm), 5mg/ml (270mOsm) and 25mg/ml (170 mOsm). The opposite eyes were injected with 0.1ml of balanced salt solution (BSS). Biomicroscopy, ophthalmoscopy, electroretinography, fluorescein angiography and histological evaluation were performed 2 hours, 4 hours, 24 hours, 7 days and 28 days after ICG injection.Results:Eyes injected with 0.5mg/ml of ICG had a delay in the B-wave latency on the first day after injection (P<0.05). Eyes injected with 5mg/ml of ICG had a delay in the B wave latency and a decrease in B-wave amplitude on the first and seventh days after injection (P<0.01); a delay in the A wave latency on the first day after injection (p<0.05) was also observed. Eyes injected with 25mg/ml of ICG had both B and A-waves amplitude and latency abnormalities during the entire follow up (P<0.01). Histology demonstrated a direct relation of increasing amount of retinal edema proportional to the progressive ICG concentrations.Conclusions:Intravitreal injection of ICG in rabbit eyes may impair retinal function and morphology proportionally to the progressive ICG dosages.
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