May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Lens Epithelial-Derived Growth Factor (LEDGF) is Expressed in Neurons Within the Mammalian Central Nervous System
Author Affiliations & Notes
  • R. Mancini
    Center for Ophthalmic Research, Brigham & Women's Hospital, Boston, MA, United States
  • L.E. Goldstein
    Center for Ophthalmic Research, Brigham & Women's Hospital, Boston, MA, United States
  • L.T. Chylack Jr
    Center for Ophthalmic Research, Brigham & Women's Hospital, Boston, MA, United States
  • Footnotes
    Commercial Relationships  R. Mancini, None; L.E. Goldstein, None; L.T. Chylack Jr, None.
  • Footnotes
    Support  MA Lion's Eye Research Fund; Brigham & Women's Hospital
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4509. doi:
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      R. Mancini, L.E. Goldstein, L.T. Chylack Jr; Lens Epithelial-Derived Growth Factor (LEDGF) is Expressed in Neurons Within the Mammalian Central Nervous System . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4509.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Lens-epithelium-derived growth factor (LEDGF) is 530 amino acid transcription factor with potent antioxidative and cytoprotective properties. LEDGF, also known as p75, shares homology with the p75 splice variant p52, heptoma-derived growth factor (HDGF), and HDGF-related proteins 1 and 2. The LEDGF gene has been sequenced and assigned (9p22.2). LEDGF may play a central role in lens epithelial cell differentiation and potently promotes survival of these cells in response to thermal, oxidative and other stresses (Singh, D.P. et al. IOVS [1999] 40:1444). Since common degenerative disorders of the brain (e.g., Alzheimer’s disease) involve oxidative damage and associated cytotoxicity, we hypothesized that LEDGF may be expressed in neurons. Here we demonstrate and characterize the expression of LEDGF within neurons within the mammalian central nervous system.Methods: We examined guinea pig and mouse primary neuronal cell cultures, as well as mouse and human brain specimen, using western blot, immunohistofluorescence, and mass spectrometry to confirm the expression, localization, and distribution of LEDGF within neurons of the central nervous system. Results: We have conclusively demonstrated that neurons and some glial elements within the mammalian central nervous system express LEDGF.Conclusions: LEDGF is expressed in neurons within the mammalial central nervous system. This transcription factor may play a role in neuronal differentiation and responses to oxidative insult associated with Alzheimer's disease.

Keywords: growth factors/growth factor receptors • gene/expression 
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