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D.P. Engel, C.E. Souza, D. Freitas, J.A. Gomes, L.B. Sousa; Microbiological Contamination of Amniotic Membrane and its Processing and Preserving Protocols’ Efficacy . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4677.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Verify the possible microbial contamination of the amniotic membrane (AM) at different times after the delivery, and investigate the antimicrobiological efficacy of the protocol used to process and preserve the AM in our service. Methods: PHASE 1: Nine AM and 10 ml of amniotic liquid from elective cesareans of patients with negative serology for Lues, Hepatitis B, C and HIV were captained. Two fragments from each membrane, from opposite sides, at different times (0, 30 and 60 minutes after the delivery) were collected and inoculated in brain heart Infusion and thioglycolate mediums. After 24hs of incubation, the solutions were replicated to blood, chocolate and Saboroud’s agar mediums for culture and identification of the microorganisms. The same procedure was done with the amniotic liquid. Phase II: Eight membranes were captained as described before, and divided in two groups: group one were processed regarding the UNIFESP’s protocol. The second group were processed using only BSA. Both samples were preserved and frozen following the same protocol for 24 h. Samples of the 2 groups were taken for culture just after the processing, preserving and frozen times. Results: All samples were contaminated in the first phase. The most prevalent microbiological contamination found was Staphilococos coagulase negative (9/9) followed by Streptococos viridans .Until now five AM came up with a negative results for both groups.in the second phase. The other three AM are still in the lab, being processed. Conclusions: All samples were contaminated in the first phase. In the second phase none of the membranes were contaminarted, showing that there is no difference between the two preservation protocols
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