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P. Iserovich, J.M. Sánchez, P.S. Reinach, J. Fischbarg; Epidermal Growth Factor Stimulates Fluid Transport in Corneal Endothelium . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4734.
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Purpose:Corneal thickness and hydration depends crucially on a balance between water leak and transport across corneal endothelium (CE). Since epidermal growth factor (EGF) stimulates fluid transport in rabbit SV40-immortalized lacrimal gland layers, we tested the effect of EGF on CE fluid transport Methods: We used deepithelialized in vitro rabbit corneal preparations. CE fluid transport activity was assessed by corneal thickness measurements with a specular microscope. Results: Discution Following thickness stabilization, EGF (20 ng/ml) application caused normal rabbit corneas (350 µm thick) to shrink after 30 min by 25 ± 0.7 µm. After 30 min exposure to hypertonic (10%) or hypotonic (-10%) conditions, application of EGF still produces shrinkage (11 ± 0.7 and 5.5 ± 0.7 µm), suggesting that the EGF effect is not on the passive leak. Experiments were done with a physiological concentration of EGF, suggesting an effect through EGF receptors in CE. Conclusions:Cytokines may play an important role in eye tissues hydration by effecting epithelial water transport activity
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