May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Macrophage Functions after Amniotic Membrane Transplantation in Mice with Herpes Stromal Keratitis
Author Affiliations & Notes
  • D. Bauer
    Department of Ophthalmology, Ophtha-Lab, Muenster, Germany
  • S. Wasmuth
    Department of Ophthalmology, University, Essen, Germany
  • H. Li
    Department of Ophthalmology, University, Essen, Germany
  • P. Hermans
    Department of Ophthalmology, University, Essen, Germany
  • N. van Rooijen
    Department of Cell Biology & Immunology, Vrije University, Amsterdam, Netherlands
  • K.P. Steuhl
    Department of Cell Biology & Immunology, Vrije University, Amsterdam, Netherlands
  • A. Heiligenhaus
    Department of Cell Biology & Immunology, Vrije University, Amsterdam, Netherlands
  • Footnotes
    Commercial Relationships  D. Bauer, None; S. Wasmuth, None; H. Li, None; P. Hermans, None; N. van Rooijen, None; K.P. Steuhl, None; A. Heiligenhaus, None.
  • Footnotes
    Support  DFG (He1877/12-1), Ernst and Berta Grimmke-Foundation
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4756. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      D. Bauer, S. Wasmuth, H. Li, P. Hermans, N. van Rooijen, K.P. Steuhl, A. Heiligenhaus; Macrophage Functions after Amniotic Membrane Transplantation in Mice with Herpes Stromal Keratitis . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4756.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: During the development of herpes stromal keratitis (HSK), macrophages enhance T cell-mediated immune response and worsen disease. Rapid improvement of HSK occurs after human amniotic membrane transplantation (AMT). In this study we determined the macrophage functions after AMT. Methods: The right corneas of BALB/c mice were infected with 105 PFU of HSV-1 (KOS strain). Animals were chosen for AMT or tarsorrhaphy that had developed ulcerating HSK on day 14 after infection. Macrophages were depleted from the eyes by corneal or subconjunctival injection of Cl2MDP-liposomes (Cl2MDP-LIP) prior to AMT; mice in the control group received PBS-liposomes (PBS-LIP). Two days after AMT, the amniotic membranes were removed. Inflammatory cell infiltration in the cornea was studied by histology and by electron microscopy. Results: Improvement of corneal ulceration and reduction of PMN infiltration was detected in the AMT group compared to the tarsorrhaphy group. After additional injection of Cl2MDP-LIP in the AMT group, inflammatory cells accumulated in the cornea compared to PBS-LIP treated group. This correlated with an increased amount of cell debris and non-viable PMNs. Conclusions: The findings suggest that macrophages infiltrating the cornea after AMT in mice with severe HSK may primarily have scavenger functions.

Keywords: herpes simplex virus • phagocytosis and killing • wound healing 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×