May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Microvascular and Inflammatory Responses to Retinal Ischemia Reperfusion Injury in Hypertensive Rats
Author Affiliations & Notes
  • F. Hirose
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • J. Kiryu
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • K. Miyamoto
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • K. Nishijima
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • S. Miyahara
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • H. Katsuta
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • H. Tamura
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • Y. Honda
    Ophthalmology & Visual Science, Kyoto Univ Graduate Sch of Med, Kyoto, Japan
  • Footnotes
    Commercial Relationships  F. Hirose, None; J. Kiryu, None; K. Miyamoto, None; K. Nishijima, None; S. Miyahara, None; H. Katsuta, None; H. Tamura, None; Y. Honda, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 4932. doi:
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    • Get Citation

      F. Hirose, J. Kiryu, K. Miyamoto, K. Nishijima, S. Miyahara, H. Katsuta, H. Tamura, Y. Honda; Microvascular and Inflammatory Responses to Retinal Ischemia Reperfusion Injury in Hypertensive Rats . Invest. Ophthalmol. Vis. Sci. 2003;44(13):4932.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: From many experimental studies in various organs, evidence is increasing in hypertensive models for inflammatory reaction with abnormal leukocyte-endothelial cell interactions. The objective of this study is to evaluate the effects of hypertension on leukocyte dynamics during retinal ischemia reperfusion injury. Methods: Transient retinal ischemia for 60 minutes was induced in spontaneously hypertensive rats (SHR) and age-matched normotensive Wister-Kyoto rats (WKY) by ligation of the optic nerve. At 4, 12, 24, and 48 hours after reperfusion, rolling leukocytes were evaluated in vivo with acridine orange digital fluorography. At 30 minutes after acridine orange injection, flat-mount of retina was prepared to evaluate leukocytes accumulated in the retinal microcirculation with fluorescence microscopy. At 14 days after reperfusion, ischemia-induced retinal damage was evaluated histologically. Results: Leukocytes rolling in SHR after reperfusion was significantly reduced; the maximam number of rolling leukocytes was reduced to 32.1% at 4 hours after reperfusion in SHR compared with WKY. Leukocytes accumulation was reduced significantly at 12 hours after reperfusion, but increased significantly at 48 hours after reperfusion in SHR. Histological examination demonstrated the significant deterioration of ischemia-induced retinal atrophy in SHR. Conclusions: Interactions between leukocytes and retinal vascular endothelium in SHR were suppressed at the early stage of retinal ischemia reperfusion injury. However, with the time, SHR showed the increased leukocyte accumulation in the retina after reperfusion and the deteriorated ischemia-induced retinal damage compared with WKY.

Keywords: ischemia • inflammation • retina 
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