May 2003
Volume 44, Issue 13
Free
ARVO Annual Meeting Abstract  |   May 2003
Survival Effect of Adenosine on Isolated Retinal Ganglion Cells in the Absence of Neurotrophins
Author Affiliations & Notes
  • C. Taki
    Bioengineering Institute, Nidek Co., Ltd., Aichi, Japan
  • M. Nakatani
    Bioengineering Institute, Nidek Co., Ltd., Aichi, Japan
  • K. Goto
    Bioengineering Institute, Nidek Co., Ltd., Aichi, Japan
  • K. Matsudaira
    Bioengineering Institute, Nidek Co., Ltd., Aichi, Japan
  • S. Nishimura
    Bioengineering Institute, Nidek Co., Ltd., Aichi, Japan
  • Footnotes
    Commercial Relationships  C. Taki, None; M. Nakatani, None; K. Goto, None; K. Matsudaira, None; S. Nishimura, None.
Investigative Ophthalmology & Visual Science May 2003, Vol.44, 5203. doi:
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      C. Taki, M. Nakatani, K. Goto, K. Matsudaira, S. Nishimura; Survival Effect of Adenosine on Isolated Retinal Ganglion Cells in the Absence of Neurotrophins . Invest. Ophthalmol. Vis. Sci. 2003;44(13):5203.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: It has been reported that adenosine, a small naturally occurring molecule, exerts a trophic effect on cultured brain cells (Lee et. al, PNAS 98:3555, 2001). The purpose of this study was to determine whether adenosine and related agonists have survival effects on cultured retinal ganglion cells (RGCs). Methods: RGCs were purified from dissociated rat retinal cells (postnatal day 6) by a modified two-step panning method (Otori, IOVS, 1998) and seeded on poly-L-lysine- and laminin-coated glass coverslips in neurobasal medium containing B27, glutamine and forskolin (the "basal medium"). Adenosine (10 and 100 µM), A2a receptor agonist CGS-21680 (2.5, 10 and 100 nM ), BDNF (40 ng/mL) or combination of BDNF, CNTF and bFGF (each of 40 ng/mL) was added to the basal medium. After 3 days of cultivation, viability of RGCs was determined by calcein-acetoxymethyl ester staining. Results: Treatment with 100 µM of adenosine increased the number of viable RGCs by 1.8-fold compared to those cultivated in the basal medium. Similar effects were also observed with 10 and 100 nM of CGS-21680 in a dose-dependent manner. Treatments with BDNF and combination of neurotrophic factors resulted in 2.1 and 3.7-fold increases, respectively. Conclusions: These results suggest that adenosine promotes the survival of RGCs in the absence of neurotrophic factors by stimulating A2a receptors.

Keywords: adenosine • ganglion cells • neuroprotection 
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