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S Doan, N Belayachi, L Ren, T Hoang-Xuan; Tear IgE Level Measurement Using Semiquantitative Methods in Children With Vernal Keratoconjunctivitis (VKC) . Invest. Ophthalmol. Vis. Sci. 2002;43(13):114.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To compare 2 semiquantitative methods of total tear IgE level measurement in VKC patients. Methods: Twenty patients (28 eyes) with VKC were included.Two commercially available kits were tested : Stallerdiag (Stallergènes, France) and Lacrytest (Adiatec, France). Tears were collected with a paper strip in the inferior conjunctival fornix, in a standardized manner. Total IgE levels were measured with Stallerdiag and Lacrytest by an ELISA technique and a colloid gold labeling technique, respectively. Lacrytest allowed an immediate assessement of the result. Threshold detection level was 2.5 kIU/L with Lacrytest and 1.7 kIU/L with Stallerdiag. Results: Results were consistent in 22/28 cases (79%) and different in 6/28 cases (21%). Both test were significantly correlated (K = 0.55). IgEs were detected in 64% and 57% of patients with Lacrytest and Stallerdiag tests, respectively. Conclusion: Stallerdiag and Lacrytest tests are comparable and easy to use for total tear IgE level assessment.
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