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P Gain, G Thuret, Y Gavet, P Turc, S Acquart, J Maugery, I Desbois, J Le Petit, L Campos; Automated Tri-image Analyser Of Stored Corneal Endothelium . Invest. Ophthalmol. Vis. Sci. 2002;43(13):132.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Endothelial examination of organ-culture-stored corneas is usually done manually and on several mosaic zones. Some banks use an image analyser that takes account of only one zone. This method is restricted by image quality, and may be inaccurate if endothelial cell density (ECD) within the mosaic is not homogeneous. We have developed an analyser that has tools for automatic error detection and correction, and can measure ECD on multiple zones of three images of the endothelial mosaic. Methods: Sixty human corneas were divided into two equal groups: group 1 with homogeneous mosaics, group 2 with heterogeneous ones. Three standard-microscopy video images of the endothelium, graded by quality, were analysed either in isolation (so-called mono-image analysis) or simultaneously (so-called tri-image analysis), with 50 or 300 endothelial cells counted. The automated analysis was compared with the manual analysis, which concerned 10 non-adjacent zones and about 300 cells. For each analysis method, failures and durations were studied according to image quality. Results: All corneas were able to undergo analysis, in about 2 or 7.5 minutes for 50 and 300 ECs respectively. The tri-image analysis did not increase analysis time and never failed, even with mediocre images. The tri-image analyses of 300 ECs were always most highly correlated with the manual count, particularly in the heterogeneous cornea group (r=0.94, p<0.001) and prevented serious count errors. Conclusion: Our analyser allows reliable and rapid analysis of ECD, even for heterogeneous endothelia and mediocre images
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