December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Expression Patterns of the Glucagon Receptor in the Chick Retina, With and Without Intravitreal Application of a Glucagon Agonist
Author Affiliations & Notes
  • MP Feldkaemper
    Department of Pathophysiology of Vision and Neuroophthalmology Section for Neurobiology of the Eye Tuebingen Germany
  • E Burkhardt
    Tubingen Germany
  • F Schaeffel
    Tubingen Germany
  • Footnotes
    Commercial Relationships   M.P. Feldkaemper, None; E. Burkhardt , None; F. Schaeffel , None. Grant Identification: German Research Council SFB 430, TP C1
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 205. doi:
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      MP Feldkaemper, E Burkhardt, F Schaeffel; Expression Patterns of the Glucagon Receptor in the Chick Retina, With and Without Intravitreal Application of a Glucagon Agonist . Invest. Ophthalmol. Vis. Sci. 2002;43(13):205.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Glucagon amacrine cells respond to growth-modulating visual stimuli with alterations in the synthesis of the transcription factor ZENK. Moreover, the amount of retinal glucagon mRNA increases during treatment with positive lenses and glucagon itself is an inhibitor of lens-induced myopia. However, the distribution and regulation of glucagon receptors in the retina are not known. Methods:(1) In situ hybridization with digoxigenin labeled riboprobes was used to demonstrate the cellular localization of glucagon receptors in the retina of 14 to 21 day-old chicks. Kidney and liver tissue served as a positive control for receptor detection. (2) Six chicks were intravitreally injected every other day with 2.5 nmol of the glucagon agonist Lys17,18,Glu21-glucagon into one eye and saline into the contralateral eye. At this concentration, the agonist inhibits lens-induced myopia development completely. After 6 days, receptor expression in both eyes was compared. Results:(1) Glucagon receptor mRNA expression was found in about 50% of the cells in the ganglion cell layer. Moreover, receptor expression was demonstrated in many cells in the inner nuclear layer. (2) The glucagon agonist Lys17,18,Glu21-glucagon led to a prominent reduction of glucagon mRNA labeling in 5 of 6 chicks. Conclusion: Even though only about 1 percent of the amacrine cells are glucagonergic, glucagon receptor expression was demonstrated in many cells in the inner nuclear layer, most of them presumably amacrine cells, and in the ganglion cell layer. These cells are, therefore, possible sites for glucagon action and perhaps also for eye growth regulation. The glucagon agonist regulates glucagon receptor expression through a negative feedback loop, a regulation which has already been described for other tissues.

Keywords: 481 myopia • 443 in situ hybridization • 557 retina: proximal(bipolar, amacrine, and ganglion cells) 
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