December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Bacterial Contamination of Paracentesis Blades Used in Cataract Surgery
Author Affiliations & Notes
  • RT Chang
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • CN Ta
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • PR Egbert
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • K Singh
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • WW Haw
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • EM Shriver
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • L Espinosa
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • DY Williams
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • MS Blumenkranz
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • H Miño de Kaspar
    Department of Ophthalmology Stanford University School of Medicine Stanford CA
  • Footnotes
    Commercial Relationships    R.T. Chang, Allergan F; C.N. Ta, Allergan F; P.R. Egbert, Allergan F; K. Singh, Allergan F; W.W. Haw, Allergan F; E.M. Shriver, Allergan F; L. Espinosa, Allergan F; D.Y. Williams, Allergan F; M.S. Blumenkranz, Allergan F; H. Miño de Kaspar, Allergan F. Grant Identification: E. E. Hills Fund, USA; Allergan, Inc; H-G Zimmerman Fund, Munich, Germany
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 363. doi:
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    • Get Citation

      RT Chang, CN Ta, PR Egbert, K Singh, WW Haw, EM Shriver, L Espinosa, DY Williams, MS Blumenkranz, H Miño de Kaspar; Bacterial Contamination of Paracentesis Blades Used in Cataract Surgery . Invest. Ophthalmol. Vis. Sci. 2002;43(13):363.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To determine the route and source of bacterial anterior chamber contamination during cataract surgery. Methods: Sterile microsurgical blades used to create a paracentesis in 117 consecutive eyes undergoing cataract surgery were immediately inoculated in blood culture broth. Forty identical microsurgical blades from sterile packages were inoculated in blood culture broth and served as negative controls. The culture media was incubated for 10 days and each bacterial isolate identified. All patients enrolled in the study received preoperative topical ofloxacin 0.3% and 5% povidone-iodine solution. Results: Nineteen of the 117 blades (16 %) used to create the paracentesis were cultured positive. None of the cultures in the control group were positive (P < .01). The most common bacteria isolated were Staphylococcus epidermidis (58 %) and Propionibacterium acnes (16 %). Conclusion: Microsurgical blades used to make the paracentesis in cataract surgery come in contact with the ocular surface, potentially introducing conjunctival bacteria into the anterior chamber.

Keywords: 317 anterior chamber • 582 small incision cataract surgery • 398 endophthalmitis 
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