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RT Chang, CN Ta, PR Egbert, K Singh, WW Haw, EM Shriver, L Espinosa, DY Williams, MS Blumenkranz, H Miño de Kaspar; Bacterial Contamination of Paracentesis Blades Used in Cataract Surgery . Invest. Ophthalmol. Vis. Sci. 2002;43(13):363.
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Purpose: To determine the route and source of bacterial anterior chamber contamination during cataract surgery. Methods: Sterile microsurgical blades used to create a paracentesis in 117 consecutive eyes undergoing cataract surgery were immediately inoculated in blood culture broth. Forty identical microsurgical blades from sterile packages were inoculated in blood culture broth and served as negative controls. The culture media was incubated for 10 days and each bacterial isolate identified. All patients enrolled in the study received preoperative topical ofloxacin 0.3% and 5% povidone-iodine solution. Results: Nineteen of the 117 blades (16 %) used to create the paracentesis were cultured positive. None of the cultures in the control group were positive (P < .01). The most common bacteria isolated were Staphylococcus epidermidis (58 %) and Propionibacterium acnes (16 %). Conclusion: Microsurgical blades used to make the paracentesis in cataract surgery come in contact with the ocular surface, potentially introducing conjunctival bacteria into the anterior chamber.
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