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T Nagamoto, S Inokawa, T Fujiwara; Inhibition Of Posterior Capsule Opacification By Aqueous Humor . Invest. Ophthalmol. Vis. Sci. 2002;43(13):430.
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Purpose:To assess the effects of normal aqueous humor on migration, proliferation, and transdifferentiation of cultured bovine lens epithelial cells, and to assess the inhibitory effect of normal aqueous humor on formation of posterior capsule opacification (PCO) in rabbit eyes implanted with a newly developed capsular adhesion preventing ring. Methods:Cell migration, proliferation, and expression of alpha-smooth muscle actin were assessed in bovine lens epithelial cells cultured on their basement membrane in MEM dissolved in pure water or MEM dissolved in normal bovine aqueous humor. We have developed a capsular adhesion preventing ring to allow circulation of aqueous humor inside the capsular bag and inhibit PCO. The inhibitory effect of this ring on formation of PCO in rabbits was assessed histologically at 8 weeks after implantation of the ring and an intraocular lens. Results:Aqueous humor inhibited migration, proliferation, and transdifferentiation (expression of alpha-smooth muscle actin) of cultured bovine lens epithelial cells. Implantation of the capsular adhesion preventing ring almost completely prevented PCO formation in rabbits. Conclusion:Normal aqueous humor inhibits migration, proliferation, and transdifferentiation of lens epithelial cells, and direct contact of normal aqueous humor with lens epithelial cells prevents PCO formation. We believe that the capsular adhesion preventing ring may be useful for the prevention of PCO in the clinical setting.
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