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MA Fragoso, V Fernandez, P Lamar, M Willcox, JM Parel; Efficiency of various drugs in the prevention of Posterior Capsular Opacification (PCO): Preliminary study . Invest. Ophthalmol. Vis. Sci. 2002;43(13):431.
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Purpose:To assess the efficiency of various drugs in the prevention of PCO. Methods:12 NZW female rabbits (1.71±0.13 kg) were utilized in this study. Lens material was removed using the Phaco-Ersatz procedure involving the removal through a micro-capsulorhexis. A mixture of viscoelastic (1.4% sodium hyaluronate, SHA, Corneal SA, Paris) and drug was injected into an empty lens capsule, allowed to remain inside for 3 min, removed, rinsed with Balanced Saline Solution (BSS) and refilled with pure SHA only. The capsulorhexis was fitted with a Mini Capsulorhexis Valve (MCV). Follow up was done at 1, 7, 14, 21, 28 and 35 days when animals were euthanized, enucleated, Miyake view and histopathology analyses performed. Rabbits were treated with one of the following: SHA (control), mitomycin-C (0.2mg/ml), EDTA (10mM and 15mM), 5-flurouracil (5-FU, 33mg/ml), and 0.003% acetic acid. Results:Upon completion of study all treated eyes had PCO and new lens material. In some eyes PCO was localized in the center of the capsule surrounded by new lens material. Anterior capsular proliferation was observed in eyes treated with 5-FU. The order of PCO appearance (earliest to latest) is as follows: control, acetic acid, mitomycin-C, 5-FU, EDTA10mM and EDTA 15mM. The earliest being POD 7, the latest POD 21. Edema around surgical incision was present only in eyes treated with acetic acid (1 POD) and EDTA 15mM (POD 1 and POD 7). Mild intraocular inflammation was present in eyes treated with EDTA 10mM and moderate in eyes treated with EDTA 15mM and acetic acid at POD 1. Conclusion:EDTA 10mM and 15mM seem to be more efficient in retarding the appearance of PCO using the fore mentioned technique. Two factors which might have contributed to PCO development in treated eyes are rabbit’s ability to regenerate lens and the high rate of Lens Epithelial Cell (LEC) proliferation in young rabbits. Additional studies are needed to determine which drugs are more efficient in preventing or delaying the appearance of PCO. Support: Henri and Flore Lesieur Foundation; the Florida Lions Eye Bank; Research to Prevent Blindness; Australian Cooperative Research Centre (CRC) Scheme, CRC for Eye Research and Technology, Sydney.
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