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R Michael, J Coppens, T van den Berg; Use of the Point Spread Function to Evaluate Forward Light Scattering During Rabbit Lens Culture . Invest. Ophthalmol. Vis. Sci. 2002;43(13):436.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To develop a method to record the optical quality of the lens during culture which is related to vision and can be compared with psychophysical measurements. To compare lens optical quality after human-eye-donor-bank-procedures against immediate lens extraction as simulated in the rabbit. Methods: The Point Spread Function (PSF) is used as a quality parameter for a quantitative description of small angle forward light scattering. PSF images were captured with a CCD camera (dynamic range 12 bit) normalized to unity and radially averaged between 0 and 7 degrees. In 6 rabbits, the eyes were enucleated 7h post-mortem and the lenses extracted 24h post-mortem. In other 6 rabbits, enucleation and lens dissection took place within 2h post-mortem. Scheimpflug and dark field photographs were taken and the PSF was measured at day 0, 1 and 2 of organ culture. Results: In both groups, lenses showed superficial haze at day 0 which disappeared at day 1 in culture. However opaque posterior fibers were visible at day 1 which corresponded to increased forward light scattering as compared with day 0. At day 2 in culture, many lenses recovered. Lenses from the 24h-post-mortem group showed on average more forward light scattering and had less good recovery then the 2h-post-mortem group. Conclusion: The measurement of the PSF allows to monitor the optical quality of lenses during culture. It gives a well defined quantitative measure of forward light scattering. The PSF has the advantage of being related to physiology and allows direct comparison with literature. Support: Marie Curie Fellowship of the European Community program Quality of Life (QLK6-CT-1999-51159)
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