December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Localization of Basal Membrane Complex Components at Lens Sutures
Author Affiliations & Notes
  • JY Lu
    Rush-Presbyterian-St Luke's Med Ctr Chicago IL
  • JR Kuszak
    Ophthalmology Pathology
    Rush-Presbyterian-St Luke's Med Ctr Chicago IL
  • KJ Al-Ghoul
    Anatomy Ophthalmology Pathology
    Rush-Presbyterian-St Luke's Med Ctr Chicago IL
  • Footnotes
    Commercial Relationships   J.Y. Lu, None; J.R. Kuszak, None; K.J. Al-Ghoul, None. Grant Identification: Support: MEBTC Research Grant (KJA) and The Dr. Bernard and Jennie M. Nelson Fund, Chicago, IL
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 457. doi:
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    • Get Citation

      JY Lu, JR Kuszak, KJ Al-Ghoul; Localization of Basal Membrane Complex Components at Lens Sutures . Invest. Ophthalmol. Vis. Sci. 2002;43(13):457.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose:To localize specific components of the Basal Membrane Complex (BMC) in elongating lens fiber ends as they approach and form posterior suture branches. Methods:Normal, juvenile (4-6 week old) Sprague-Dawley rat lenses (n=24) were fixed immediately following enucleation, embedded in 2 % agar, then sectioned with a vibrating knife microtome. Three-four serial sections, 100µm thick, were cut parallel to the equatorial plane beginning at the posterior pole. F-actin was localized with phalloidin-FITC; myosin, beta-1 integrin and N-cadherin were localized by immunofluorescent techniques. Specimens were visualized on a laser scanning confocal microscope. Negative controls were performed to assure specificity of immuno-labeling. Results:As fibers approached the posterior suture branches, F-actin labeling was seen to be predominately localized to the periphery of the BMC. This was consistent with F-actin localization in more laterally-positioned fiber ends. At sutures, F-actin in the BMC was rearranged into numerous small profiles. Furthermore, in fibers which had detached from the capsule, a band of intense F-actin staining was noted adjacent to the sutures. Myosin was present in the BMC as a diffuse plaque which filled the fiber ends. Although beta-I integrin was also distributed throughout the BMC, it was overlain by instances of bright, punctate fluorescence. Directly adjacent to and at the suture branches, immuno-fluorescence for beta-1 integrin was markedly reduced. As expected, initial N-cadherin characterization showed a strong localization to the periphery of the BMC. Conclusion:F-actin and integrin components of the BMC undergo a major rearrangement in the final stages of migration and in detachment from the capsule to form suture branches. Further, the intense staining of F-actin in detached fibers adjacent to the sutures suggests that lens fibers may have a structure analogous to the terminal web of other epithelia.

Keywords: 471 microscopy: confocal/tunneling • 434 immunohistochemistry • 316 animal model 

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