December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Decreased Expression of Ribosomal Proteins in Age-related Human Cataract
Author Affiliations & Notes
  • JR Hawse
    Biology West Virginia University Morgantown WV
  • W Zhang
    Biology West Virginia University Morgantown WV
  • Q Huang
    The Jules Stein Eye Institute UCLA Medical School Los Angeles CA
  • N Sheets
    Biology West Virginia University Morgantown WV
  • KM Miller
    The Jules Stein Eye Institute UCLA Medical School Los Angeles CA
  • J Horwitz
    The Jules Stein Eye Institute UCLA Medical School Los Angeles CA
  • M Kantorow
    Biology West Virginia University Morgantown WV
  • Footnotes
    Commercial Relationships   J.R. Hawse, None; W. Zhang, None; Q. Huang, None; N. Sheets, None; K.M. Miller, None; J. Horwitz, None; M. Kantorow, None. Grant Identification: NIH Grant EY13022
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 469. doi:
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    • Get Citation

      JR Hawse, W Zhang, Q Huang, N Sheets, KM Miller, J Horwitz, M Kantorow; Decreased Expression of Ribosomal Proteins in Age-related Human Cataract . Invest. Ophthalmol. Vis. Sci. 2002;43(13):469.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: We have previously identified multiple differentially expressed genes between cataract and normal human lenses that function in growth control, Ca++-homeostasis and metal-detoxification pathways. Here, we sought to identify additional differentially expressed genes between cataract and normal lenses. Methods: RNA was isolated from 90 cataract (70% mixed, 20% nuclear, 5% cortical and 2% PSC) and 41 normal lens epithelia and analyzed for differential gene expression by RT-PCR differential display. Transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing. The expression levels of identified clones were confirmed and the expression levels of functionally similar candidate genes were evaluated between cataract and normal lenses by quantitative RT-PCR. The spatial expression patterns of identified genes were also examined in micodissected normal lenses. Results: Multiple transcripts exhibited significant differences in gene expression. One transcript, exhibiting a 5-fold decrease in expression between cataract and normal lenses, was identified as ribosomal protein L21. Three additional ribosomal proteins, L15, L13a, and L7a, also exhibited significantly decreased expression in cataract compared to normal lenses. By contrast, the levels of elongation factor 1 alpha 1 (EF1a1) and initiation factor 4E (eIF4E) were unchanged. Identical levels of RPL21 were detected throughout the normal lens epithelium and high levels were also detected in lens fibers. Conclusions: The data suggest that decreased expression of ribosomal proteins may direct decreased protein synthesis in age-related cataract and they point to protein synthesis pathways as being important for the maintenance of lens transparency.

Keywords: 338 cataract • 417 gene/expression • 528 proteins encoded by disease genes 
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