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GJ Naaman, GA Peyman, AA Moshfeghi, M Genaidy, DM Moshfeghi, F Ghahramani, S Yoneya, Y Ito, T Sodeyama; Transpupillary Thermotherapy and NPe6 Photodynamic Therapy in Normal Rabbit Fundi: Threshold Parameters and Effect of Ordering . Invest. Ophthalmol. Vis. Sci. 2002;43(13):580.
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Purpose: To determine the transpupillary thermotherapy (TTT) and mono-L-aspartyl chlorin e6 (NPe6) photodynamic therapy (PDT) parameters required to induce funduscopically and angiographically visible lesions on the pigmented and non-pigmented (albino) rabbit fundus and to determine if the treatment sequence is relevant. Methods: Two pigmented rabbits and 5 albino rabbits were used in this study. In each group, an 810-nm diode laser with slit-lamp delivery system was used to provide a TTT spot size of 3.0 mm during 1 minute of laser irradiance. Power for TTT was 120 mW in pigmented rabbits and 1250 mW in albino rabbits. For both pigmented and albino rabbits, PDT power was 3 mW for either 3 or 4 seconds with a 1.2 mm spot size. The ordering of therapy was also altered (TTT or PDT alone, TTT before PDT, or PDT before TTT). Results: For pigmented rabbits (at the above parameters), treatment order affected the appearance of lesions both on fundus examination and by angiography such that the greatest effect was seen when TTT was applied before PDT and the least effect occurred with PDT alone. A similar pattern was noted with albino rabbits; however, TTT alone did not result in the appearance of lesions (these applications were made at subthreshold levels). The greatest effect for the albino subset was noted when PDT (3 mW, 4 seconds) was performed prior to TTT (1250 mW), although substantial lesions were also noted when this ordering was reversed. Conclusion: For both pigmented and albino rabbits, the most visible lesions were noted when NPe6 PDT was performed prior to TTT. The combination of PDT and TTT produced a synergistic effect in both albino and pigmented rabbit fundi.
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