December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
The Effects of Neurotrophins on Myocilin Expression by Human Trabecular Meshwork Cells
Author Affiliations & Notes
  • X Liu
    Cell Biology & Genetics Univ N Texas Health Sci Center Fort Worth TX
  • AF Clark
    Glaucoma Research Alcon Research Ltd Fort Worth TX
  • RJ Wordinger
    North Texas Eye Research Institute University of North Texas Health Science Center Fort Worth TX
  • Footnotes
    Commercial Relationships   X. Liu, None; A.F. Clark, Alcon Research Ltd. E; R.J. Wordinger, Alcon Research Ltd. F.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1028. doi:
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      X Liu, AF Clark, RJ Wordinger; The Effects of Neurotrophins on Myocilin Expression by Human Trabecular Meshwork Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1028.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Neurotrophins are a family of survival factors for neurons that are expressed by trabecular meshwork cells {Wordinger et al., 2000; IOVS; 41(12):3833-41} and found in the aqueous humor {Chundru et al., 2000; IOVS; 41[4], S326}. Previous studies have shown that the expression of the glaucomatous gene MYOC is up-regulated by glucocorticoid {Clark et al., 2001; IOVS; 42(8):1769-80} and transforming growth factor-beta1 {Tamm et al., 1999; IOVS; 40(11):2577-82}. The purpose of our study is to determine whether neurotrophins (BDNF, NGF, NT-3 and NT-4) regulate human TM myocilin expression and secretion. Methods: Cultured HTM cells were treated with BDNF (50 ng/ml), NGF (50 ng/ml), NT-3 (50 ng/ml) and NT-4 (50 ng/ml) in serumless Ham's nutrient mixture F-10 media for 48 hours, respectively. RT-PCR and Western blot analysis were used to determine the effects of neurotrophins on myocilin mRNA, protein expressions, and secretion. Results: Both RT-PCR and Western blot analysis demonstrated the up-regulation of myocilin expression and secretion in the HTM cells upon BDNF treatment. Variable results were seen with NGF, NT-3 and NT-4 treatment with respect to MYOC expression. Conclusion: To our knowledge, this is the first report that a neurotrophin can exert regulatory effect on myocilin expression in cultured HTM cells. This raises the distinct possibility that paracrine/autocrine signaling via neurotrophins exists within the human trabecular meshwork.

Keywords: 601 trabecular meshwork • 423 growth factors/growth factor receptors • 417 gene/expression 
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