December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Toxicity of Preserved and Unpreserved Antiglaucoma Eyedrops on Human Cultured Trabecular Cells
Author Affiliations & Notes
  • P Hamard
    Dpt of Ophthalmology Quinze-Vingts Hospital AP-HP University Paris V Paris France
  • C Debbasch
    Pharmacy Faculty Toxicology Laboratory University Paris V France
  • F Brignole
    Dpt of Immunohematology Ambroise-Paré Hospital University Paris V Paris France
  • C Baudouin
    Dpt of Ophthalmology Quinze-Vingts Hospital AP-HP University Paris V Paris France
  • Footnotes
    Commercial Relationships   P. Hamard, None; C. Debbasch, None; F. Brignole, None; C. Baudouin, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1035. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      P Hamard, C Debbasch, F Brignole, C Baudouin; Toxicity of Preserved and Unpreserved Antiglaucoma Eyedrops on Human Cultured Trabecular Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1035.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To compare the toxicity of short-time application of antiglaucoma eyedrops with and without benzalkonium chloride preservative (respectively BAC+ and BAC-) in an in vitro model of human trabecular cells. Material and Methods: Human immortalized trabecular cells lines (Abbot Clark, Alcon laboratories, Fort Worth, TX) were treated for 15 min. with 0.5% timolol BAC+ and BAC-, 0.25% betaxolol suspension BAC+ and BAC-, 0.005% latanoprost, and 0.01% BAC in 1:10 and 1:100 dilutions. Cell size and the expression of an apoptotic marker Apo 2.7 were evaluated 24 hours after by flow cytometry. Results: All tested drugs in a 1:10 and 1:100 dilutions, except 0.5% timolol BAC-, induced significant trabecular cell size decrease as compared to controls (p<0.05), more pronounced with 0.001% BAC (p<10-4). Preserved eye drops induced significant cell size decrease as compared to nonpreserved eyedrops in a 1:10 dilution (p<0.04). When increasing drug concentration from 1:100 to 1:10, trabecular cell size significantly decreased only with preservated eyedrops (p<0.05). Apo 2.7 expression was significantly increased with 0.001% BAC (p<10-4) and 0.0001% BAC (p<0.005). 0.005% latanoprost in a 1:10 dilution induced significant Apo 2.7 overexpression as compared to 0.5% timolol BAC- and 0.25% betaxolol BAC- in a 1:10 dilution (p<0.05). Conclusion: Benzalkonium chloride induces in vitro cytotoxic damage to trabecular cells with some characteristics of apoptosis.

Keywords: 601 trabecular meshwork • 514 pharmacology • 323 apoptosis/cell death 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×