December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Neuroprotective Effect of a TOPS-ester on Retinal Ischemia-Reperfusion Injury
Author Affiliations & Notes
  • J Sebag
    Doheny Eye Institute Keck School of Medicine USC Los Angeles CA
  • S Garg
    Doheny Eye Institute Keck School of Medicine USC Los Angeles CA
  • L Tong
    Doheny Eye Institute Keck School of Medicine USC Los Angeles CA
  • AA Sadun
    Doheny Eye Institute Keck School of Medicine USC Los Angeles CA
  • L Ma
    SynZyme Technologies LLC Irvine CA
  • TT Lam
    Doheny Eye Institute Keck School of Medicine USC Los Angeles CA
  • Footnotes
    Commercial Relationships    J. Sebag, SynZyme Technologies, LLC C; S. Garg, None; L. Tong, None; A.A. Sadun, None; L. Ma, SynZyme Technologies, LLC E; T.T. Lam, SynZyme Technologies, LLC F.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 776. doi:
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    • Get Citation

      J Sebag, S Garg, L Tong, AA Sadun, L Ma, TT Lam; Neuroprotective Effect of a TOPS-ester on Retinal Ischemia-Reperfusion Injury . Invest. Ophthalmol. Vis. Sci. 2002;43(13):776.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: This study evaluates the neuroprotective effect of a TOPS-ester (ethyl-2,2,6,6-tetramethylpiperidylidene succinate (ETOPS)), a novel caged nitric oxide, on retinal ganglion cell (RGC) survival in an animal model of retinal ischemia-reperfusion injury. Methods: Ischemia-reperfusion injury to the retina of 55-60 day old male Lewis albino rats was induced by cannulating the anterior chamber with a 27 gauge needle and infusing a saline solution to elevate intraocular pressure to 110 mmHg for 60 minutes. Retinal ischemia was confirmed by observing whitening of the anterior segment and blanching of the retina. The study was performed with 3 groups of animals: saline infusion (n=8), saline + vehicle (n=8), and saline + 0.1 mM ETOPS (n=8). ETOPS or vehicle was infused into the eye during the ischemic insult. The animals were allowed to recover for 7 days and were then euthanized. The eyes were enucleated and the retinas were removed and flat-mounted for staining with cresyl violet and RGC counting. Results: Both in the posterior and the peripheral retina, the ETOPS-treated group showed significantly (p< 0.02; Student's t-test) more cells in the RGC layer than the vehicle treated group. Conclusion: ETOPS has a significant neuroprotective effect on retinal cells subjected to retinal ischemia-reperfusion injury.

Keywords: 489 neuroprotection • 557 retina: proximal(bipolar, amacrine, and ganglion cells) • 321 antioxidants 
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