December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effects of Granulocyte Colony Stimulating Factor (G-CSF) on Retinal Leukocyte and Erythrocyte Flux in the Human Retina
Author Affiliations & Notes
  • G Fuchsjaeger-Mayrl
    Clinical Pharmacology University of Vienna Vienna Austria
  • M Malec
    Clinical Pharmacology University of Vienna Vienna Austria
  • L Schmetterer
    Clinical Pharmacology University of Vienna Vienna Austria
  • Footnotes
    Commercial Relationships   G. Fuchsjaeger-Mayrl, None; M. Malec, None; L. Schmetterer, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 840. doi:
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      G Fuchsjaeger-Mayrl, M Malec, L Schmetterer; Effects of Granulocyte Colony Stimulating Factor (G-CSF) on Retinal Leukocyte and Erythrocyte Flux in the Human Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):840.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:The blue field entoptic technique has been introduced more than 20 years ago to quantify perimacular white blood cell flux. However, a final confirmation that the perceived corpuscles represent leukocytes is still lacking. Methods:The study design was randomized, placebo-controlled, double masked with 2 parallel groups. Fifteen healthy male subjects received a single dose of granulocyte colony stimulating factor (G-CSF, 300 µg) and 15 other subjects received placebo. The following parameters were assessed at baseline, 12 minutes and 8 hours after administration: retinal white blood cell flux using the blue field entoptic technique, retinal blood velocities using bi-directional laser Doppler velocimetry, retinal venous diameter using the Zeiss retinal vessel analyzer, blood pressure and pulse rate using automated oscillometry and pulse oxymetry, respectively. Results:After 12 minutes G-CSF reduced total leukocyte count from 5.6 ± 1.4 G/L at baseline to 2.0 ± 0.4 G/L. This was paralleled by a 35 ± 11% decrease in retinal white blood cell density. After 8 hours G-CSF increased total leukocyte counts to 20.0 ± 4.4 G/L. Again, this increase in circulating leukocytes was reflected by an increase in retinal white blood cell density (110 ± 48%). All effects were significant at p<0.001. By contrast, none of the other hemodynamic parameters was changed by administration of G-CSF. Conclusion:Our results clearly indicate that the blue field entoptic technique assesses leukocyte movement in the perimacular capillaries of the retina. Moreover, white blood cell density appears to adequately reflect the number of circulating leukocytes within the retinal microvasculature. Since white and red blood cell flux can easily be uncoupled in the retina the blue field entoptic technique should not be used to assess retinal perfusion.

Keywords: 331 blood supply • 554 retina • 514 pharmacology 
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