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C Gias, M Jones, D Keegan, P Adamson, J Greenwood, RD Lund, J Martindale, D Johnston, J Mayhew, P Coffey; Optical Imaging Demonstrates Amelioration of Cortical Visual Function in Dystrophic Rats Following Transplantation of Human Immortalised RPE Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):921.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: The aim of this series of experiments was to use optical imaging technology to investigate visual cortical function in dystrophic Royal College of Surgeons (RCS) rats following sub-retinal transplantation of human immortalised RPE cells. Methods: 3-4 week old dystrophic RCS rats received sub-retinal injections of a human immortalised RPE cell line (h1RPE7). At 5 months of age animals were anaesthetised and the skull overlying the right visual cortex was thinned to translucency. A 12 bit CCD camera was used to image the changes in remitted illumination from visual cortex. A number of visual stimuli were presented to the rats left eye: flickering full screen flash plus horizontal and vertical drifting gratings of 0.05, 0.15 and 0.4 cycles/degree. Data were averaged over 30 trials. Following location of activity, spectroscopy was performed to further examine the hemodynamic response to stimulation. Furthermore, visually evoked potentials were recorded from both surface and layer IV of the visual cortex. Results: In transplanted animals responses could be detected to full screen flash and various gratings. Single wavelength imaging detected cortical darkening following stimulus presentation, which indicated an increase in blood volume characteristic of neural activity. The presence of hemodynamic responses was confirmed by imaging spectroscopy. Surface and layer IV visually evoked potentials were also recorded from the 'optically active' region. Control dystrophic animals did not show this pattern of visual cortical function. Conclusion: These data confirm the efficacy of human immortalised RPE transplants and suggest optical imaging may be a useful tool for visual cortical functional assessment.
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