December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Corneal Response to Pseudomonas Aeruginosa Infection in the Absence of IL-10
Author Affiliations & Notes
  • NA Cole
    Cooperative Research Centre for Eye Research and Technology University of New South Wales Sydney Australia
  • M Krockenberger
    Veterinary Anatomy and Pathology University of Sydney Sydney Australia
  • F Stapleton
    Cooperative Research Centre for Eye Research and Technology University of New South Wales Sydney Australia
  • AJ Husband
    Veterinary Anatomy and Pathology University of Sydney Sydney Australia
  • MD P Willcox
    Cooperative Research Centre for Eye Research and Technology University of New South Wales Sydney Australia
  • Footnotes
    Commercial Relationships   N.A. Cole, None; M. Krockenberger, None; F. Stapleton, None; A.J. Husband, None; M.D.P. Willcox, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 942. doi:
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    • Get Citation

      NA Cole, M Krockenberger, F Stapleton, AJ Husband, MD P Willcox; Corneal Response to Pseudomonas Aeruginosa Infection in the Absence of IL-10 . Invest. Ophthalmol. Vis. Sci. 2002;43(13):942.

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Abstract

Abstract: : Purpose: To explore the role of IL-10 in the cornea during Pseudomonas aeruginosa corneal infection by examining the effects of the absence of IL-10 on the pathogenesis of this disease. Method: IL-10 gene knockout and wildtype mice were challenged by topical application of P. aeruginosa to wounded corneas. Corneas were examined clinically, microbiologically and histologically over 7 days. Additionally, cytokine and myeloperoxidase levels in corneas were determined. Results: The IL-10 gene knockout mice showed less severe corneal disease than the wild type mice, however, they had more extensive vascularisation of the cornea at 7 days. IL-10 gko mice had approximately five-fold reduction of the bacterial load at 7 days post-challenge compared to the wild type mice, with corresponding changes in chemokine, cytokine and VEG-F levels. Conclusion: The absence of IL-10, a pleiotropic cytokine with anti-inflammatory effects, leads to more effective control of bacterial infection of the cornea, but more extensive vascularisation. Local administration of anti-IL-10 antibody early in the infectious process may improve outcomes during this destructive disease of the cornea.

Keywords: 316 animal model • 380 cytokines/chemokines • 531 Pseudomonas 
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