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A Harris, L Kagemann, C Amin, R Migliardi, T Ciulla, O Arend; A New Spectrographic Digital Fundus Oxymeter: Effect of Hyperoxia on Retinal Oxygen Tension . Invest. Ophthalmol. Vis. Sci. 2002;43(13):990.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To determine the ability of a newly developed spectrographic digital fundus oxymeter to detect differences in oxygen tension in retinal vessels induced by 100% oxygen breathing (hyperoxia). Methods: Thirteen healthy subjects (26.5 ± 3.8years) were evaluated before and after 100% oxygen breathing. Three measurements were obtained while breathing room air, focusing on the major temporal retinal vessels adjacent to the optic disc. After five minutes of 100% oxygen breathing, two additional measurements were taken. Oxygen breathing was maintained during all measurements. The fundus was illuminated by white light, from which two frequencies were quantified: 605nm (oxygen sensitive) and 586nm (oxygen insensitive). After calibrating the images to optical densities, the optical density ratio (ODR) between oxygen sensitive and insensitive wavelengths was calculated. Baseline and hyperoxic ODR in the temporal arteries and veins were compared by Wilcoxon signed rank paired comparisons (p< 0.05 = statistical significance). Results: Hyperoxia significantly increased venous ODR by 9.4% (p = 0.009). Venous oxygen tension was significantly less than arterial oxygen tension at baseline (p=0.04). Hyperoxia eliminated this difference. Conclusion: It is possible to measure changes in arterial and venous oxygen tensions in the human eye with the spectrographic digital fundus oxymeter. The ability to measure oxygen extraction by the neuroretinal rim and retinal tissue with this instrument will open up new horizons of research into the vascular pathophysiology of glaucoma and other ocular diseases.
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