December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
A Simplified Method for the Quantitation of Laser-Induced Choroidal Neovascularization in the Rat
Author Affiliations & Notes
  • CK Spee
    Ophthalmology Doheny Eye Institute Keck School of Medicine at the University of Southern California Los Angeles CA
  • E Barron
    Los Angeles CA
  • DR Hinton
    Los Angeles CA
  • SJ Ryan
    Los Angeles CA
  • Footnotes
    Commercial Relationships   C.K. Spee, None; E. Barron , None; D.R. Hinton , None; S.J. Ryan , None. Grant Identification: NIH grants EY03040 and EY01545, Research to Prevent Blindness and the Arnold and Mabel Beckman Found
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1266. doi:
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    • Get Citation

      CK Spee, E Barron, DR Hinton, SJ Ryan; A Simplified Method for the Quantitation of Laser-Induced Choroidal Neovascularization in the Rat . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1266.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To analyze the three-dimensional microvascular architecture of laser-induced choroidal neovascularization (CNV) in rats and the associated cellular behaviors associated with outer retinal wound healing. Methods: Experimental CNV was induced by laser photocoagulation-mediated disruption of Bruch’s membrane in Long Evans rats. Vessels were localized by staining with the FITC-conjugated endothelial specific Griffonia simplicifolia isolectin B4 (GS-I-B4; Vector Laboratories, Burlingame CA). Vessel staining on the whole-mount RPE-choroid-sclera complex was optically sectioned with confocal laser scanning microscopy (CLSM), (Zeiss LSM 510 software) and the digitised data set(s) were further processed and analysed with the Zeiss LSM topography program for volume quantitation. Results: This is a reproducible, simplified method that is representative of the neovascular area in laser-induced CNV. In addition, it is cost effective and requires less exhaustive hours, as does the perfusion method. Conclusion: Three-dimensional reconstruction of a CNV lesion by CLSM enabled a qualitative and quantitative analysis of the whole CNV lesion in rats. This method may contribute to the study of the cellular and molecular mechanisms of CNV and the development of new therapies against CNV.

Keywords: 346 choroid: neovascularization • 471 microscopy: confocal/tunneling • 454 laser 

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