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S Hoffmann, U Friedrichs, A Niggemann, W Eichler, P Wiedemann; Angiogenic Activity of Pyruvic Acid on Choroidal Endothelial Cells In Vitro . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1299.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To determine the effect of pyruvic acid on choroidal endothelial cell (CEC) proliferation and cell signalling, key components in the process of new vessel formation in exudative age-related macular degeneration (AMD). Pyruvic acid is discussed to prevent cells from oxidative stress. Methods: CECs were exposed to pyruvic acid, an endproduct of glycolysis in a concentration range of 1, 5 and 10 mM in vitro. After 3 days of exposure, the CEC proliferation was estimated by MTT. The cytokines bFGF and VEGF were used in a concentration of 10 µM as a control in these assays. Furthermore, the CECs were exposed to the MAP-kinase inhibitor PD98059 in addition with pyruvic acid to investigate the relevance of the MAP-kinase pathway for pyruvic acid induced effects. Results:Pyruvic acid strongly enhanced the proliferative response of CECs estimated by MTT. A concentration of 1 mM pyruvic acid revealed a 50% increase in the MTT assay. A concentration of 5 mM pyruvic acid revealed an increase of 85% and a concentration of 10 mM revealed an increase of 100% in the MTT assay. The MAP-kinase inhibitor PD98059 inhibited in a concentration of 4 µM in all samples the proliferative response induced by pyruvic acid. Conclusion: Pyruvic acid, a major end product of metabolism is involved in the proliferative response of CECs by modulating the MAP-kinase pathway. Pyruvic acid may be an additional denominator of CEC neovascularization in exudative AMD.
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