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S He, M Jin, V Woerpel, Y Sekine, SJ Ryan, DR Hinton; Connective Tissue Growth Factor expression in choroidal neovascular membranes and its regulation by VEGF . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1303.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To evaluate the expression of connective tissue growth factor (CTGF) in choroidal neovascular membranes (CNVM) of patients with age-related macular degeneration (AMD), the regulation of CTGF in vitro by vascular endothelial growth factor (VEGF) and the effect of CTGF on choroidal endothelial cell (CEC) function. Methods: Using immunohistochemical methods, we analyzed CTGF expression in ten surgically excised AMD-related CNVMs. The effects of CTGF on attachment, migration, proliferation and tube formation of bovine CEC were studied in cultures stimulated with CTGF. The effect of VEGF on the expression of CTGF protein was evaluated using Western blot. Results: Vascularized human CNVM showed strong CTGF immunoreactivity. Double staining revealed co-localization of CTGF with RPE cells and CEC. CTGF induced a significant increase in attachment, migration and tube formation in CEC. CTGF protein was upregulated in CEC by stimulation of VEGF. Conclusion: The expression of CTGF in human CNVM and its upregulation by VEGF suggest that CTGF may play a role in the pathogenesis of AMD-related CNV.
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