December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Sialoadhesin Function in Th1 Cell-mediated Experimental Autoimmune Uveoretinitis
Author Affiliations & Notes
  • H Jiang
    Ophthalmology Univ of Aberdeen Medical Sch Aberdeen United Kingdom
  • C Jones
    The Wellcome Trust Biocentre at Dundee School of Life Sciences University of Dundee Dundee United Kingdom
  • E Muckersie
    Ophthalmology Univ of Aberdeen Medical Sch Aberdeen United Kingdom
  • M Robertson
    Ophthalmology Univ of Aberdeen Medical Sch Aberdeen United Kingdom
  • M Vinson
    Neurology CEDD GSK New Frontiers Science Park North Harlow United Kingdom
  • PR Crocker
    The Wellcome Trust Biocentre at Dundee School of Life Sciences University of Dundee Dundee United Kingdom
  • JV Forrester
    Ophthalmology Univ of Aberdeen Medical Sch Aberdeen United Kingdom
  • Footnotes
    Commercial Relationships   H. Jiang, None; C. Jones, None; E. Muckersie, None; M. Robertson, None; M. Vinson, None; P.R. Crocker, None; J.V. Forrester, None. Grant Identification: No
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1533. doi:
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    • Get Citation

      H Jiang, C Jones, E Muckersie, M Robertson, M Vinson, PR Crocker, JV Forrester; Sialoadhesin Function in Th1 Cell-mediated Experimental Autoimmune Uveoretinitis . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1533.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To investigated the role of sialoadhesin (Sn) molecule in the development of a Th1 cell-mediated experimental autoimmune uveoretinitis (EAU) by using Sn-deficient (Sn-/-)mice. Method: Sn +/- and -/- C57BL/6 mice were immunised with uveitogenic antigen- IRBP peptide emulsified in CFA (s.c.), together with pertussis toxin (i.p.). At day 12, 16 and 26, eyes were removed for EAU grading. Also peritoneal macrophages from naïve or immunised mice were collected and cultured with IFN-γ+TNF-α to detect nitric oxide production. Results: The incidence and severity of EAU in Sn -/- mice were reduced compared to Sn+/- mice on day 12 and 16, but had reached similar levels in both groups of mice by day 26 post immunisation. In addition, Sn -/- macrophages from naïve and immunised mice produced less nitric oxide (NO) in the presence of IFN-γ+TNF-α. Conclusion: The delay in disease onset and the reduction in NO production suggest that macrophage - host cell interactions may be influenced by Sn possibly at the stage of antigen presentation to primed T cells and /or recognition and clearance of apoptotic cells, effete erythrocytes and opsonized cells.

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