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H Jiang, C Jones, E Muckersie, M Robertson, M Vinson, PR Crocker, JV Forrester; Sialoadhesin Function in Th1 Cell-mediated Experimental Autoimmune Uveoretinitis . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1533.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To investigated the role of sialoadhesin (Sn) molecule in the development of a Th1 cell-mediated experimental autoimmune uveoretinitis (EAU) by using Sn-deficient (Sn-/-)mice. Method: Sn +/- and -/- C57BL/6 mice were immunised with uveitogenic antigen- IRBP peptide emulsified in CFA (s.c.), together with pertussis toxin (i.p.). At day 12, 16 and 26, eyes were removed for EAU grading. Also peritoneal macrophages from naïve or immunised mice were collected and cultured with IFN-γ+TNF-α to detect nitric oxide production. Results: The incidence and severity of EAU in Sn -/- mice were reduced compared to Sn+/- mice on day 12 and 16, but had reached similar levels in both groups of mice by day 26 post immunisation. In addition, Sn -/- macrophages from naïve and immunised mice produced less nitric oxide (NO) in the presence of IFN-γ+TNF-α. Conclusion: The delay in disease onset and the reduction in NO production suggest that macrophage - host cell interactions may be influenced by Sn possibly at the stage of antigen presentation to primed T cells and /or recognition and clearance of apoptotic cells, effete erythrocytes and opsonized cells.
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