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T Oshida, S Kobayakawa, A Tuji, K Tanaka, Y Okajima, T Tochikubo; In vitro Adherence of Staphylococcus epidermidis and Enterococcus faecalis to Intraocular Lenses . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1590.
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Purpose: In this study, in vitro adherence of clinically relevant ocular isolate of Staphylococcus epidermidis FK-MA and Enterococcus faecalis KO-S1 to various intraocular lenses (IOL) was investigated. Methods: Bacterial adherence was tested on one-piece PMMA, one-piece silicone, three-piece silicone (two-type), one-piece acrylic, three-piece acrylic (two-type) and one-piece hydrogel IOLs. Eight IOLs (one of each type) were placed in different test plate containing bacterial suspension (10(5) cfu/ml) and incubated at 35 degrees C. At different times (24 hours and 48 hours), each IOL was removed and rinsed, then vortexed to separate the adhered bacteria. Quantitative cultures were performed. Results: After 24 hours incubation, The mean bacterial adherence of E. faecalis to the one-piece silicone was 5.37 cfu/IOL (log), to all of other IOL was 6.07-6.92 cfu/IOL (log). After 48 hours incubation, The mean bacterial adherence of E. faecalis to the three-piece acrylic was 6.48-6.64 cfu/IOL (log), to the one-piece acrylic was 6.48 cfu/IOL (log), three-piece silicone was 5.91-6.52 cfu/IOL (log), one-piece hydrogel was 6.04 cfu/IOL (log), one-piece PMMA was 4.52 cfu/IOL (log), one-piece silicone was 1.43 cfu/IOL (log). There were no differences in vitro adherence between S. epidermidis and E. faecalis . Conclusion: Results suggest that in vitro adherence of S. epidermidis and E. faecalis to IOLs is influenced by IOL materials. Acrylic IOLs appeared to be more permissive than other IOLs.
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