December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Detection of Telomerase Activity in Ocular Surface Tumors
Author Affiliations & Notes
  • M Ueno
    Ophthalmology Kyoto Prefectural Univ of Med Kyoto Japan
  • W Adachi
    Ophthalmology Kyoto Prefectural Univ of Med Kyoto Japan
  • S Kawasaki
    Ophthalmology Kyoto Prefectural Univ of Med Kyoto Japan
  • C Mochida
    Ophthalmology Kyoto Prefectural Univ of Med Kyoto Japan
  • S Kinoshita
    Ophthalmology Kyoto Prefectural Univ of Med Kyoto Japan
  • Footnotes
    Commercial Relationships   M. Ueno, None; W. Adachi, None; S. Kawasaki, None; C. Mochida, None; S. Kinoshita, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1659. doi:
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    • Get Citation

      M Ueno, W Adachi, S Kawasaki, C Mochida, S Kinoshita; Detection of Telomerase Activity in Ocular Surface Tumors . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1659.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:The ribonucleoprotein telomerase maintains telomere length and ensures chromosomal stability in supporting continued cell growth. Telomerase activation is found in human malignant tumors and stem cells. There are no reports on the telomerase activity of sqamous cell carcinomas and conjunctival intraepithelial neoplasia (CIN) in the ocular surface. This study investigated telomerase activity in these tumors in comparision with normal limbal epithelium. Methods:Using telomeric repeat amplification protocol (TRAP) assay, we examined the telomerase activity of 1 squamous cell carcinoma, 2 CIN and limbal epithelia. Normal conjunctival epithelium and corneal epithelium obtained from bullous keratopathy were used as controls. Results:Telomerase activity was found in these tumors and in limbal epithelium, but no telomerase activity was detected in normal conjunctival epithelium or corneal epithelium from bullous keratopathy. Telomerase activity in these tumors was far higher than that in limbal elithelium. There was correlation between telomerae activity in these tumors and a clinical grade of malignancy. Conclusion:As in other tumors, telomerase activity was detected in ocular surface tumors. this activity reflected the grade of malignancy, and might be useful as a biomarker in the ocular surface tumors. The fact that telomerase activity was detected in limbal epithelium strongly suggests the concentrated presence of corneal epithelial stem cells.

Keywords: 372 cornea: epithelium • 610 tumors • 476 molecular biology 
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