December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effects of Cyclosporin A on Human ß-Defensin in Corneal Epithelial Cells
Author Affiliations & Notes
  • K Terai
    Ophthalmology Kyoto Prefectural Univ Med Kyoto Japan
  • Y Sano
    Ophthalmology Kyoto Prefectural Univ Med Kyoto Japan
  • S Kawasaki
    Ophthalmology Kyoto Prefectural Univ Med Kyoto Japan
  • S Kinoshita
    Ophthalmology Kyoto Prefectural Univ Med Kyoto Japan
  • Footnotes
    Commercial Relationships   K. Terai, None; Y. Sano, None; S. Kawasaki, None; S. Kinoshita, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1660. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      K Terai, Y Sano, S Kawasaki, S Kinoshita; Effects of Cyclosporin A on Human ß-Defensin in Corneal Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1660.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: It has been suggested that human ß-defensin (HBD) plays a role in the defense against microbial infection, and it has recently been shown that the cornea produces HBDs. We have reported that dexamethasone reduce HBD-1 mRNA expression in corneal epithelial cells. In this series of experiments, we wished to examine the effects of cyclosporin A, which is clinically often administered as immunosuppressive agaents, on mRNA expression of HBDs by corneal epithelial cells in vitro. Methods: Corneal epithelial cells from an immortalized corneal epithelial cell line (SV40) were cultured at a concentration of 6×104 cells/cm2 in culture medium (SHEM medium) in 6 well dish. After 72hrs, when the cultures reached confluence, cells were harvested and total RNA was extracted. Using semi-quantitive PCR and competitive PCR, HBD-1 and HBD-2 mRNA expression were examined. In some cultures, dexamethasone (10-5, 10-7M) or cyclosporin A (1, 10-1 mg/l) was added to the cultures at 48 hours. The level of HBDs mRNA expression by corneal epithelium cultured with or without dexamethasone or cyclosporin A was analyzed by both semi-quantitive PCR which is a conventional method and introduced amplified fragment length polymorphism (iAFLP) which is a more exact method. Results: Semi-quantitive PCR and iAFLP revealed that dexamethasone suppressed only HBD-1 mRNA expression in corneal epithelial cells in a dose-dependent manner, however cyclosporin A suppressed only HBD-2 mRNA expression in corneal epithelial cells in a dose-dependent manner. Conclusion: These results suggest that the regulations of HBD-1 and HBD-2 are completely deferent between dexamethasone and cysclosporin A. We imply that ocular surface defensive abilities against bacterial infection may differ between the administration of dexamethasone and cyclosporine A.

Keywords: 372 cornea: epithelium 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×