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EM Espana, M Grueterich, SC G Tseng; Immunofluorescent Study on Basement Membrane Proteins and Epithelial Survival Following Brief Ethanol Exposure Like Lasek . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1686.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:To determine the viability of cultured human corneal epithelial cells and the anatomical cleavage plane after 20 sec exposure to 20% ethanol to create an epithelial flap in a manner similar to LASEK. Methods:Tryptan blue staining was used to determine the viability of cultured corneal cultured cells. Immunofluorescent staining using monoclonal antibodies against laminin 5 and integrins α3ß1 and α6ß4 was performed to determine the anatomical location of the cleavage plane in an epithelial flap created in donor human eyes. Results:There was no difference in cell viability between the exposed and non-exposed cultured human corneal epithelial cells (p=0.422), indicating that a brief exposure to ethanol was not toxic. Immnunofluorescence staining revealed positive patchy staining for laminin 5 and integrins in both the lifted flap and the remaining corneal basement membrane, indicating that the cleavage plane was within the corneal basement membrane. Conclusion:Brief exposure to ethanol does not produce immediate death of corneal epithelial cells. The cleavage plane of a corneal epithelial flap located within the epithelial basement membrane is important to the understanding of LASEK in refractive surgery.
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