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J Kong, C Donmoyer, K Lai, P Gouras; The Effect of Temperature on the Murine ERG . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1781.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To study effects of body temperature on the murine electroretinogram (ERG). Methods:The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 380 C. Results:ERGs of approximately normal configuration were recorded at body temperatures ranging from 30 to 380C. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude with temperature was 150 microvolts/degree. At 330 C it was about 600 microvolts. At 370 C maximum b-wave amplitude was about 1200 microvolts. At any given temperature the ERG remained stable. If the temperature was changed, ERG amplitude changed rapidly following a linear relationship. Conclusion:The murine ERG is extremely sensitive and linear to temperature changes with a Q10 of about 5. The ERG is therefore a sensitive retinal thermometer. Other mammalian ERGs would probably behave similarly if body temperature could be changed as rapidly as the mouse. In order to follow the murine ERG accurately over time, continuous recording of rectal temperature seems imperative.
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