December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Gene Expression Profiling of Angiogenic and Anti-Angiogenic Factors Between Corneal and Conjunctival Epithelial Cells
Author Affiliations & Notes
  • E Sekiyama
    Dept of Ophthalmology Kyoto Prefectural Univ of Med Kyoto City Japan
  • S Kawasaki
    Dept of Ophthalmology Kyoto Prefectural Univ of Med Kyoto City Japan
  • K Endo
    Dept of Ophthalmology Kyoto Prefectural Univ of Med Kyoto City Japan
  • C Mochida
    Dept of Ophthalmology Kyoto Prefectural Univ of Med Kyoto City Japan
  • T Nakamura
    Dept of Ophthalmology Kyoto Prefectural Univ of Med Kyoto City Japan
  • S Kinoshita
    Dept of Ophthalmology Kyoto Prefectural Univ of Med Kyoto City Japan
  • Footnotes
    Commercial Relationships   E. Sekiyama, None; S. Kawasaki, None; K. Endo, None; C. Mochida, None; T. Nakamura, None; S. Kinoshita, None. Grant Identification: Support: the Japanese Ministry of Education (12470367)
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1865. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      E Sekiyama, S Kawasaki, K Endo, C Mochida, T Nakamura, S Kinoshita; Gene Expression Profiling of Angiogenic and Anti-Angiogenic Factors Between Corneal and Conjunctival Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1865.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Corneal avascularity is thought to be maintained by unknown factors produced by corneal epithelial cells and/or keratocytes. The purpose of this study is to elucidate the role of corneal epithelial cells in the maintenance of corneal avascularity. Methods: Human samples in this study included 4 fresh corneal buttons obtained at PKP surgery, 3 preserved corneas purchased from an US eye bank, 6 conjunctival epithelia obtained from normal volunteers by brush cytology, and 1 cultivated corneal epithelial sheet. RNAs extracted from epithelial cells of these samples were subjected to subsequent iAFLP analysis, a kind of competitive RT-PCR. Genes examined were angiogenic factors such as VEGF, angiogenin, IL-8, erythropoietin and TNF alpha 2, and anti-angiogenic factors such as angiogenin inhibitor, GRO-beta, IP-10, NK4 and thrombospondin 3. Results: The gene expression of VEGF, IL-8, and angiogenin, known as angiogenic factors, were markedly down-regulated in corneal and cultivated corneal samples with apparent segregation. Paradoxically, GRO-beta and angiogenin inhibitor, known as anti-angiogenic factors, were also down-regulated in corneal samples. Notably, VEGF, known to be a key factor in angiogenesis, was less expressed in fresh corneal and cultivated corneal epithelium than in fresh conjunctival epithelium, but remarkably up-regulated in preserved corneal epithelium. Conclusion: Down-regulation of several angiogenic and anti-angiogenic factors suggests complex interaction among these factors in precisely regulating corneal avascularity.  

Keywords: 417 gene/expression • 372 cornea: epithelium • 365 conjunctiva 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×